Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | dependent on, the DTT cencentration influences the target specificity of ART2 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged ART2 as maltose-binding fusion protein in Escherichia coli strain BL21(DE3), expression of the membrane-bound ART2 in COS1 cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-ribose | partial competitive inhibition | Mus musculus | |
Triton X-100 | detergent solubilization of cell membranes specifically abolishes ADP-ribosylation of high MW histones | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | ART2 is attached to the cell surface by a glycosylphosphatidylinositol anchor in T-cells | Mus musculus | 9986 | - |
extracellular | serum | Mus musculus | - |
- |
membrane | - |
Mus musculus | 16020 | - |
additional information | the membrane or solution environment of ART2 plays a pivotal role in determining its substrate specificity | Mus musculus | - |
- |
soluble | - |
Mus musculus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone + NAD+ | Mus musculus | isozyme ART2 is active with high and low molecular weight histones, membrane-bound and PI-PLC released ART2 preferentially ADP-ribosylate high or low MW histones, respectively | Nomega-[(2'-phospho-ADP)-D-ribosyl]-histone-L-arginine + nicotinamide + H+ | - |
? | |
additional information | Mus musculus | protein mono-ADP-ribosylation is a reversible post-translational modification that plays a role in regulation of cellular activities specific amino acid of the acceptor protein, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
isozyme ART2 | - |
Purification (Comment) | Organism |
---|---|
recombinant membrane-bound His-tagged ART2 maltose-binding fusion protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion echange chromatography, and amylose resin affnity chromataography, the maltose-binding protein is cleaved off | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
serum | - |
Mus musculus | - |
T-lymphocyte | ART2 is a T-cell-specific transferase | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone + NAD+ | isozyme ART2 is active with high and low molecular weight histones, membrane-bound and PI-PLC released ART2 preferentially ADP-ribosylate high or low MW histones, respectively | Mus musculus | Nomega-[(2'-phospho-ADP)-D-ribosyl]-histone-L-arginine + nicotinamide + H+ | - |
? | |
additional information | protein mono-ADP-ribosylation is a reversible post-translational modification that plays a role in regulation of cellular activities specific amino acid of the acceptor protein, overview | Mus musculus | ? | - |
? | |
additional information | ART2, in the absence of histone acceptor, has NAD+ glycohydrolase activity, overview, serum proteins are ADP-ribosylated in a thiol-specific manner, soluble recombinant ART2 lacking the GPI anchor shows a different histone specificity than does native cell-bound ART2, the membrane or solution environment of ART2 plays a pivotal role in determining its substrate specificity | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ART | - |
Mus musculus |
mono-ADP-ribosyltransferase | - |
Mus musculus |
NAD:arginine ADP-ribosyltransferase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Mus musculus |