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Literature summary for 2.4.2.31 extracted from

  • Vogelsgesang, M.; Aktories, K.
    Exchange of glutamine-217 to glutamate of Clostridium limosum exoenzyme C3 turns the asparagine-specific ADP-ribosyltransferase into an arginine-modifying enzyme (2006), Biochemistry, 45, 1017-1025.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli Hathewaya limosa

Protein Variants

Protein Variants Comment Organism
E219Q site-directed mutagenesis, almost inactive mutant Hathewaya limosa
Q217E site-directed mutagenesis, the mutation results in inhibition of the enzyme's ADP-ribosyltransferase activity toward RhoA, the mutant protein is still capable of NAD+-binding and possesses NAD+ glycohydrolase activity, the mutant is capable of ADP-ribosylation of poly-arginine but not poly-asparagine Hathewaya limosa
R151A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Hathewaya limosa
R61A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Hathewaya limosa
R86A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Hathewaya limosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Hathewaya limosa

Organism

Organism UniProt Comment Textmining
Hathewaya limosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off by thrombin Hathewaya limosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme exhibits ADP-ribosyltransferase and NAD+ glycohydrolase activity, modeling of interaction od substrate and catalytic residues, overview Hathewaya limosa ?
-
?
RhoA + NAD+ the enzyme modifies the low-molecular-mass GTPase RhoA specifically at Asn41 Hathewaya limosa Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoA + nicotinamide
-
?
RhoB + NAD+ the enzyme modifies the low-molecular-mass GTPase RhoB specifically at Asn41 Hathewaya limosa Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoB + nicotinamide
-
?
RhoC + NAD+ the enzyme modifies the low-molecular-mass GTPase RhoC specifically at Asn41 Hathewaya limosa Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoC + nicotinamide
-
?
soybean trypsin inhibitor + NAD+
-
Hathewaya limosa Nomega-(ADP-D-ribosyl)-L-arginine-soybean trypsin inhibitor + nicotinamide
-
?
wild-type exoenzyme C3 + NAD+ the recombinant mutant Q217E enzyme modifies the recombinant wild-type enzyme at Arg86 Hathewaya limosa Nomega-(ADP-D-ribosyl)-L-arginine86-wild-type exoenzyme C3 + nicotinamide
-
?

Subunits

Subunits Comment Organism
More the enzyme contains an ADP-ribosylating turn-turn motif, i.e. ARTT-motif, with the key residue Q217, that is involved in substrate specificity and recognition Hathewaya limosa

Synonyms

Synonyms Comment Organism
asparagine-specific ADP-ribosyltransferase
-
Hathewaya limosa
exoenzyme C3
-
Hathewaya limosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37 assay at Hathewaya limosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Hathewaya limosa