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Literature summary for 2.4.2.14 extracted from

  • Bera, A.K.; Chen, S.; Smith, J.L.; Zalkin, H.
    Temperature-dependent function of the glutamine phosphoribosylpyrophosphate amidotransferase ammonia channel and coupling with glycinamide ribonucleotide synthetase in a hyperthermophile (2000), J. Bacteriol., 182, 3734-3739.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aquifex aeolicus

Inhibitors

Inhibitors Comment Organism Structure
ADP 4.1 mM, 50% inhibition Aquifex aeolicus
AMP 6.12 mM, 50% inhibition Aquifex aeolicus
GDP 8.5 mM, 50% inhibition Aquifex aeolicus
GMP IC50: 7.9 mM Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.87
-
5-phospho-alpha-D-ribose 1-diphosphate at 90°C Aquifex aeolicus
3
-
L-glutamine at 90°C Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
Aquifex aeolicus 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
both glutaminase and amidotransferase activity Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
glutaminase half-life: 25 h Aquifex aeolicus
80
-
glutaminase half-life: 65 min Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5 glutaminase activity Aquifex aeolicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.9
-
IC50: 7.9 mM Aquifex aeolicus GMP