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Literature summary for 2.4.2.14 extracted from

  • Nagy, M.; Reichert, U.; Ribet, A.M.
    Regulation of purine metabolism in Schizosaccharomyces pombe. IV. Variations in the stability and kinetic parameters of amidophosphoribosyltransferase depending on growth phase and growth conditions (1974), Biochim. Biophys. Acta, 370, 85-95.
    View publication on PubMed
Search for more literature for 2.4.2.14

General Stability

General Stability Organism
stability profile depends on growth rate and growth phase of cell culture, PMSF improves stability Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
AMP 2.7 mM and 1.2 mM, 50% inhibition of stable and unstable enzyme preparation respectively Schizosaccharomyces pombe
GMP 0.44 mM and 0.25 mM, 50% inhibition of stable and unstable enzyme preparation respectively Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 mutant unstable enzyme, gel filtration Schizosaccharomyces pombe
360000
-
 wild-type stable enzyme, gel filtration Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
 wild type strain 972h- and mutant strain ade 2h- devoid of adenylosuccinate synthase
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin, ammonium sulfate, Sephadex g-200 Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
-
 Schizosaccharomyces pombe

Storage Stability

Storage Stability Organism
3°C, crude mutant enzyme extract, inactivation overnight, partially purified mutant enzyme: inactivation within 72 h, PMSF stabilizes Schizosaccharomyces pombe
3°C, crude or partially purified wild-type enzyme, at least 72 h stable Schizosaccharomyces pombe
PMSF stabilizes during storage Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
 Schizosaccharomyces pombe 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 wild-type, stable enzyme from cells of mid-exponential growth phase, t1/2: 34 min, from cells of stationary growth phase, t1/2: 2-5 min, mutant enzyme, t1/2: 2 min, in the presence of ADP or adenosine 3 min, and 4 min in the presence of IMP Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 unstable enzyme Schizosaccharomyces pombe
8
-
 stable enzyme Schizosaccharomyces pombe

pH Range

pH Minimum pH Maximum Comment Organism
7 9 stable enzyme, approx. 65% and unstable enzyme approx. 70% of maximal activity at pH 7, stable enzyme, approx. 75% and unstable enzyme approx. 65% of maximal activity at pH 9 Schizosaccharomyces pombe