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Literature summary for 2.4.2.14 extracted from
- Kinetic, physical, and regulatory properties of amidophosphoribosyltransferase (1981), Adv. Enzyme Regul., 19, 215-231.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | 0.48 | 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens |  |
| 0.47 | - |
5-phospho-alpha-D-ribose 1-diphosphate | adenocarcinoma 755 | Homo sapiens | |
| 0.57 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Mus musculus | |
| 1 | 4.5 | L-glutamine | - |
Homo sapiens | |
| 1.24 | 1.5 | L-glutamine | - |
Rattus norvegicus | |
| 1.8 | - |
L-glutamine | adenocarcinoma 755 | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Columba livia | |
| Fe | loss of activity after oxidation of the iron-sulfur center | Mus musculus | |
| Fe | enzyme contains a [4Fe-4S] cluster | Cricetulus griseus | |
| Fe | enzyme contains a [4Fe-4S] cluster | Bacillus subtilis | |
| Fe | enzyme contains a [4Fe-4S] cluster | Mus musculus | |
| Fe | enzyme contains a [4Fe-4S] cluster | Homo sapiens | |
| additional information | - |
Escherichia coli | |
| additional information | 4Fe-4S-cluster | Cricetulus griseus | |
| additional information | 4Fe-4S-cluster | Bacillus subtilis | |
| additional information | 4Fe-4S-cluster | Mus musculus | |
| additional information | 4Fe-4S-cluster | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the smaller molecular weight form of the liver enzyme is observed when incubated with purine nucleotides, the smaller one when incubated with phosphoribosyldiphosphate | Columba livia |
| additional information | - |
enzymes from human placenta, Chinese hamster fibroblasts and mouse liver exist in two molecular weight forms, the larger one is observed when incubated with purine nucleotides, the smaller one when incubated with phosphoribosyldiphosphate | Cricetulus griseus |
| additional information | - |
enzymes from human placenta, Chinese hamster fibroblasts and mouse liver exist in two molecular weight forms, the larger one is observed when incubated with purine nucleotides, the smaller one when incubated with phosphoribosyldiphosphate | Mus musculus |
| additional information | - |
enzymes from human placenta, Chinese hamster fibroblasts and mouse liver exist in two molecular weight forms, the larger one is observed when incubated with purine nucleotides, the smaller one when incubated with phosphoribosyldiphosphate | Homo sapiens |
| 127000 | - |
liver small form | Mus musculus |
| 133000 | - |
placenta small form, large form is converted to small form by incubation with phosphoribosyldiphosphate | Homo sapiens |
| 170000 | - |
placenta large form, in the presence of AMP or GMP | Homo sapiens |
| 215000 | - |
gel filtration | Rattus norvegicus |
| 215000 | - |
only one enzyme form | Rattus norvegicus |
| 270000 | - |
placenta, large form, small form is converted to large form by incubation with purine nucleotides, large form presumably catalytically inactive | Homo sapiens |
| 292000 | - |
liver, large form | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Rattus norvegicus | - |
L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Cricetulus griseus | first reaction in de-novo pathway of purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Bacillus subtilis | first reaction in de-novo pathway of purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Mus musculus | first reaction in de-novo pathway of purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Escherichia coli | first reaction in de-novo pathway of purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Homo sapiens | first reaction in de-novo pathway of purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Columba livia | first reaction in de-novo pathway of purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Columba livia | - |
- |
- |
| Cricetulus griseus | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| amido- and aminotransferase activity is lost after exposure to oxygen | Mus musculus |
| oxygen sensitive enzyme | Cricetulus griseus |
| oxygen sensitive enzyme | Mus musculus |
| oxygen sensitive enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adenocarcinoma cell | adenocarcinoma 755 | Homo sapiens | - |
| erythroleukemia cell | - |
Mus musculus | - |
| fibroblast | - |
Cricetulus griseus | - |
| leukocyte | - |
Homo sapiens | - |
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Columba livia | - |
| placenta | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Cricetulus griseus | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Bacillus subtilis | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Mus musculus | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Escherichia coli | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Rattus norvegicus | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Columba livia | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | glutamine binding site distinct from NH3-site | Homo sapiens | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Rattus norvegicus | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | first reaction in de-novo pathway of purine biosynthesis | Cricetulus griseus | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | first reaction in de-novo pathway of purine biosynthesis | Bacillus subtilis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | first reaction in de-novo pathway of purine biosynthesis | Mus musculus | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | first reaction in de-novo pathway of purine biosynthesis | Escherichia coli | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | first reaction in de-novo pathway of purine biosynthesis | Homo sapiens | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | first reaction in de-novo pathway of purine biosynthesis | Columba livia | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Cricetulus griseus | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Bacillus subtilis | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Mus musculus | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Escherichia coli | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Columba livia | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
| NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | NH3-binding site is distinct from glutamine-site | Homo sapiens | 5-phospho-beta-D-ribosylamine + diphosphate | - |
? | |
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