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Literature summary for 2.4.2.1 extracted from

  • Zhou, X.; Szeker, K.; Janocha, B.; Böhme, T.; Albrecht, D.; Mikhailopulo, I.A.; Neubauer P.
    Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides (2013), FEBS J., 280, 1475-1490.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
adenosine 80°C, pH 7.0 Aeropyrum pernix
0.137
-
Inosine 80°C, pH 7.0 Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, the higher oligomeric state of the enzyme is catalytically active, whereas the monomeric ApMTAP is inactive, SDS-PAGE Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YDC0
-
-
Aeropyrum pernix DSM 11879 Q9YDC0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-amino-2'-deoxyadenosine + phosphate
-
Aeropyrum pernix adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
?
2'-amino-2'-deoxyadenosine + phosphate
-
Aeropyrum pernix DSM 11879 adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
?
2'-amino-2'-deoxyinosine + phosphate
-
Aeropyrum pernix hypoxanthine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
?
2'-deoxy-2'-fluoroadenosine + phosphate
-
Aeropyrum pernix adenine + 2-deoxy-2-fluoro-alpha-D-arabinofuranose 1-phosphate
-
?
2'-deoxyadenosine + phosphate
-
Aeropyrum pernix adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2'-deoxyadenosine + phosphate
-
Aeropyrum pernix DSM 11879 adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2'-deoxyinosine + phosphate
-
Aeropyrum pernix hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2'-deoxyinosine + phosphate
-
Aeropyrum pernix DSM 11879 hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
9-(2-deoxy-2-fluoro-alpha-D-arabinofuranosyl)adenine + phosphate
-
Aeropyrum pernix adenine + 2-deoxy-2-fluoro-alpha-D-arabinofuranose 1-phosphate
-
?
adenosine + phosphate
-
Aeropyrum pernix adenine + alpha-D-ribose 1-phosphate
-
?
adenosine + phosphate
-
Aeropyrum pernix DSM 11879 adenine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Aeropyrum pernix hypoxanthine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Aeropyrum pernix DSM 11879 hypoxanthine + alpha-D-ribose 1-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 27000, the higher oligomeric state of the enzyme is catalytically active, whereas the monomeric ApMTAP is inactive, SDS-PAGE Aeropyrum pernix

Synonyms

Synonyms Comment Organism
5'-methythioadenosine phosphorylase
-
Aeropyrum pernix
ApMTAP
-
Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Aeropyrum pernix
99
-
activity of the enzyme increases exponentially at increased reaction temperatures up to the highest temperature tested (99°C) Aeropyrum pernix

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 99 80°C: about 35% of maximal activity, activity of the enzyme increases exponentially at increased reaction temperatures up to the highest temperature tested (99°C) Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life: 69 h Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
adenosine 80°C, pH 7.0 Aeropyrum pernix
23
-
Inosine 80°C, pH 7.0 Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Aeropyrum pernix

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
Inosine 80°C, pH 7.0 Aeropyrum pernix
210
-
adenosine 80°C, pH 7.0 Aeropyrum pernix