Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
adenosine | 80°C, pH 7.0 | Aeropyrum pernix | |
0.137 | - |
Inosine | 80°C, pH 7.0 | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, the higher oligomeric state of the enzyme is catalytically active, whereas the monomeric ApMTAP is inactive, SDS-PAGE | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YDC0 | - |
- |
Aeropyrum pernix DSM 11879 | Q9YDC0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-amino-2'-deoxyadenosine + phosphate | - |
Aeropyrum pernix | adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate | - |
? | |
2'-amino-2'-deoxyadenosine + phosphate | - |
Aeropyrum pernix DSM 11879 | adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate | - |
? | |
2'-amino-2'-deoxyinosine + phosphate | - |
Aeropyrum pernix | hypoxanthine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxy-2'-fluoroadenosine + phosphate | - |
Aeropyrum pernix | adenine + 2-deoxy-2-fluoro-alpha-D-arabinofuranose 1-phosphate | - |
? | |
2'-deoxyadenosine + phosphate | - |
Aeropyrum pernix | adenine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyadenosine + phosphate | - |
Aeropyrum pernix DSM 11879 | adenine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyinosine + phosphate | - |
Aeropyrum pernix | hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyinosine + phosphate | - |
Aeropyrum pernix DSM 11879 | hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
9-(2-deoxy-2-fluoro-alpha-D-arabinofuranosyl)adenine + phosphate | - |
Aeropyrum pernix | adenine + 2-deoxy-2-fluoro-alpha-D-arabinofuranose 1-phosphate | - |
? | |
adenosine + phosphate | - |
Aeropyrum pernix | adenine + alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | - |
Aeropyrum pernix DSM 11879 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Aeropyrum pernix | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Aeropyrum pernix DSM 11879 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, the higher oligomeric state of the enzyme is catalytically active, whereas the monomeric ApMTAP is inactive, SDS-PAGE | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
5'-methythioadenosine phosphorylase | - |
Aeropyrum pernix |
ApMTAP | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Aeropyrum pernix |
99 | - |
activity of the enzyme increases exponentially at increased reaction temperatures up to the highest temperature tested (99°C) | Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 99 | 80°C: about 35% of maximal activity, activity of the enzyme increases exponentially at increased reaction temperatures up to the highest temperature tested (99°C) | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 69 h | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
adenosine | 80°C, pH 7.0 | Aeropyrum pernix | |
23 | - |
Inosine | 80°C, pH 7.0 | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aeropyrum pernix |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
Inosine | 80°C, pH 7.0 | Aeropyrum pernix | |
210 | - |
adenosine | 80°C, pH 7.0 | Aeropyrum pernix |