Application | Comment | Organism |
---|---|---|
synthesis | synthesis of ribavirin by the enzyme and comparison with Escherichia coli enzyme. A cold-adapted Pseudoalteromonas enzyme shows approximately 15 degrees lower optimum temperature and 1.80fold higher catalytic efficiency at 37°C within substrate inosine than its homolog from Escherichia coli | Pseudoalteromonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
Inosine | pH 7.6, 37°C | Pseudoalteromonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, calculated and SDS-PAGE | Pseudoalteromonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. | C8CPR9 | - |
- |
Pseudoalteromonas sp. XM2107 | C8CPR9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine + phosphate | - |
Pseudoalteromonas sp. | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Pseudoalteromonas sp. XM2107 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, calculated and SDS-PAGE | Pseudoalteromonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Pseudoalteromonas sp. |
50 | - |
complete inactivation | Pseudoalteromonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
84.1 | - |
Inosine | pH 7.6, 37°C | Pseudoalteromonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pseudoalteromonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | stable for at least 2 h at 37°C | Pseudoalteromonas sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
215 | - |
Inosine | pH 7.6, 37°C | Pseudoalteromonas sp. |