Crystallization (Comment) | Organism |
---|---|
modeling of the inhibitors 1-((2-pyrrolidine-1-yl)ethyl)uracil and 3-((2-pyrrolidine-1-yl)ethyl)uracil into the active site of the protein, PDB entry 1NW4, by superposition of the uracil ring and the purine ring of Immucillin-H. Both compounds can fit adequately in the active site. In both cases the uracil base is predicted to be oriented in the nucleoside binding pocket by a pi-stacking similar to the purine base of Immucillin-H. The N1 of the uracil base in 1-((2-pyrrolidine-1-yl)ethyl)uracil and the N3 of the uracil base in 3-((2-pyrrolidine-1-yl)ethyl)uracil roughly superimpose with the C4 of Immucillin-H | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-((2-pyrrolidine-1-yl)ethyl)uracil | inhibits both enzymic activity and growth of Plasmodium falciparum | Plasmodium falciparum | |
3-((2-pyrrolidine-1-yl)ethyl)uracil | inhibits both enzymic activity and growth of Plasmodium falciparum | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
Inosine | pH 7.5, 25°C | Plasmodium falciparum | |
0.085 | - |
uridine | pH 7.5, 25°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8I3X4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine + phosphate | - |
Plasmodium falciparum | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Plasmodium falciparum | uracil + alpha-D-ribose 1-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
3-((2-pyrrolidine-1-yl)ethyl)uracil | pH 7.5, 25°C | Plasmodium falciparum | |
0.027 | - |
1-((2-pyrrolidine-1-yl)ethyl)uracil | pH 7.5, 25°C | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.306 | - |
uridine | pH 7.5, 25°C | Plasmodium falciparum | |
74 | - |
Inosine | pH 7.5, 25°C | Plasmodium falciparum |