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Literature summary for 2.4.2.1 extracted from

  • Luo, W.; Liu, Y.; Zhu, X.; Zhao, W.; Huang, L.; Cai, J.; Xu, Z.; Cen, P.
    Cloning and characterization of purine nucleoside phosphorylase in Escherichia coli and subsequent ribavirin biosynthesis using immobilized recombinant cells (2011), Enzyme Microb. Technol., 48, 438-444.
    View publication on PubMed

Application

Application Comment Organism
synthesis use of agar from four different polymer carriers as a suitable matrix for whole recombinant cell entrapment. Although the immobilization process reduces the substrate affinity and catalytic efficiency of recombinant cells, it can significantly enhance the stability and reusability of these cells. The immobilized whole cell biocatalyst is applied to produce ribavirin, as a model nucleoside synthesis reaction showing relative high productivity of rabavirin and quick reaction time Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ABP8
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + phosphate 54.7% of the activity with 2'-deoxyinosine Escherichia coli adenine + 2-deoxy-alpha-D-ribose 1-phosphate
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?
2'-deoxyguanosine + phosphate 83.2% of the activity with 2'-deoxyinosine Escherichia coli guanine + 2-deoxy-alpha-D-ribose 1-phosphate
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?
2'-deoxyinosine + phosphate
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Escherichia coli hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
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?
adenosine + phosphate 59.9% of the activity with 2'-deoxyinosine Escherichia coli adenine + alpha-D-ribose 1-phosphate
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?
guanosine + phosphate 58.7% of the activity with 2'-deoxyinosine Escherichia coli guanine + alpha-D-ribose 1-phosphate
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?
inosine + phosphate 83% of the activity with 2'-deoxyinosine Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
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?
additional information no substrate: xanthosine, uridine, 2'-deoxythymidine, cytidine Escherichia coli ?
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?