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Literature summary for 2.4.2.1 extracted from

  • Wielgus-Kutrowska, B.; Breer, K.; Hashimoto, M.; Hikishima, S.; Yokomatsu, T.; Narczyk, M.; Dyzma, A.; Girstun, A.; Staro?, K.; Bzowska, A.
    Trimeric purine nucleoside phosphorylase: exploring postulated one-third-of-the-sites binding in the transition state (2012), Bioorg. Med. Chem., 20, 6758-6769.
    View publication on PubMed
Search for more literature for 2.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
calorimetric titration of recombinant calf PNP complexed with immucillin H. Stoichiometry shows three immucillin molecules per enzyme trimer, enzyme does not show negative cooperativity. One-third-of-the-sites binding does not occur for trimeric enzyme Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine
-
 Bos taurus
cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid with (hypoxanthine-9-yl)methyl aglycone Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0134
-
 Inosine wild-type, pH not specified in the publication, temperature not specified in the publication Bos taurus
0.0168
-
 7-methylguanosine wild-type, pH not specified in the publication, temperature not specified in the publication Bos taurus
0.05
-
 7-methylguanosine mutant F159W, pH not specified in the publication, temperature not specified in the publication Bos taurus
0.07
-
 7-methylguanosine mutant F200W, pH not specified in the publication, temperature not specified in the publication Bos taurus
0.245
-
 Inosine mutant F159W, pH not specified in the publication, temperature not specified in the publication Bos taurus
0.326
-
 Inosine mutant F200W, pH not specified in the publication, temperature not specified in the publication Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methylguanosine + phosphate
-
 Bos taurus 7-methylguanine + alpha-D-ribose 1-phosphate
-
 ?
guanosine + phosphate
-
 Bos taurus guanine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Bos taurus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0069
-
 9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine wild-type, pH not specified in the publication, temperature not specified in the publication Bos taurus
0.063
-
 cis-1,1-difluoro-2-(tetrahydro-3-piranozyl)ethylphosphonic acid wild-type, pH not specified in the publication, temperature not specified in the publication Bos taurus