Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine | multisubstrate analogue inhibitor. Upon titration of the recombinant enzyme, endothermic as well as exothermic signals are obtained. Part of the recombinant enzyme forms a complex with its product, hypoxanthine, although hypoxanthine was not present at any purification stage except for its natural occurrence in Escherichia coli cells. Binding of hypoxanthine is accompanied with a large negative change of the free enthalpy, and therefore the replacement of hypoxanthine by 9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine yields a positive heat signal | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |