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Literature summary for 2.4.2.1 extracted from

  • Breer, K.; Wielgus-Kutrowska, B.; Girstun, A.; Staro?, K.; Hashimoto, M.; Hikishima, S.; Yokomatsu, T.; Bzowska, A.
    Overexpressed proteins may act as mops removing their ligands from the host cells: a case study of calf PNP (2010), Biochem. Biophys. Res. Commun., 391, 1203-1209.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine multisubstrate analogue inhibitor. Upon titration of the recombinant enzyme, endothermic as well as exothermic signals are obtained. Part of the recombinant enzyme forms a complex with its product, hypoxanthine, although hypoxanthine was not present at any purification stage except for its natural occurrence in Escherichia coli cells. Binding of hypoxanthine is accompanied with a large negative change of the free enthalpy, and therefore the replacement of hypoxanthine by 9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine yields a positive heat signal Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-