Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.1 extracted from

  • Ducati, R.G.; Santos, D.S.; Basso, L.A.
    Substrate specificity and kinetic mechanism of purine nucleoside phosphorylase from Mycobacterium tuberculosis (2009), Arch. Biochem. Biophys., 486, 155-164.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
guanosine pH 7.0, 25°C Mycobacterium tuberculosis
0.015
-
2'-deoxyguanosine pH 7.0, 25°C Mycobacterium tuberculosis
0.029
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
Mycobacterium tuberculosis
0.033
-
7-Methylinosine pH 7.0, 25°C Mycobacterium tuberculosis
0.04
-
Inosine pH 7.0, 25°C Mycobacterium tuberculosis
0.068
-
2'-deoxyinosine pH 7.0, 25°C Mycobacterium tuberculosis
0.427
-
phosphate cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C Mycobacterium tuberculosis
0.427
-
phosphate cosubstrate 7-methylinosine, pH 7.0, 25°C Mycobacterium tuberculosis
0.583
-
phosphate cosubstrate inosine, pH 7.0, 25°C Mycobacterium tuberculosis
1.131
-
phosphate cosubstrate 2'-deoxyinosine, pH 7.0, 25°C Mycobacterium tuberculosis
1.345
-
phosphate cosubstrate guanosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.349
-
phosphate cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate steady-state ordered bi bi kinetic mechanism, in which phosphate binds first followed by 2'-deoxyguanosine, and ribose 1-phosphate dissociates first followed by guanine. A general acid is essential for both catalysis and 2'-deoxyguanosine binding, and deprotonation of a group abolishes phosphate binding. Product release contributes to the rate-limiting step Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyguanosine + phosphate
-
Mycobacterium tuberculosis guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?
2'-deoxyinosine + phosphate
-
Mycobacterium tuberculosis 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
-
Mycobacterium tuberculosis alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
?
7-methylguanosine + phosphate
-
Mycobacterium tuberculosis 7-methylguanine + alpha-D-ribose 1-phosphate
-
?
7-methylinosine + phosphate
-
Mycobacterium tuberculosis 7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
?
guanosine + phosphate
-
Mycobacterium tuberculosis guanine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Mycobacterium tuberculosis hypoxanthine + alpha-D-ribose 1-phosphate
-
?
additional information enzyme is more specific to natural 6-oxopurine nucleosides and synthetic compounds, and does not catalyze the phosphorolysis of adenosine Mycobacterium tuberculosis ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
phosphate cosubstrate guanosine, pH 7.0, 25°C Mycobacterium tuberculosis
4
-
guanosine pH 7.0, 25°C Mycobacterium tuberculosis
4.9
-
phosphate cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C Mycobacterium tuberculosis
4.9
-
phosphate cosubstrate inosine, pH 7.0, 25°C Mycobacterium tuberculosis
5.1
-
2'-deoxyguanosine pH 7.0, 25°C Mycobacterium tuberculosis
5.4
-
Inosine pH 7.0, 25°C Mycobacterium tuberculosis
6.9
-
phosphate cosubstrate 2'-deoxyinosine, pH 7.0, 25°C Mycobacterium tuberculosis
7.5
-
2'-deoxyinosine pH 7.0, 25°C Mycobacterium tuberculosis
13.3
-
phosphate cosubstrate 7-methylinosine, pH 7.0, 25°C Mycobacterium tuberculosis
17.3
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
Mycobacterium tuberculosis
17.9
-
phosphate cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C Mycobacterium tuberculosis
18
-
7-Methylinosine pH 7.0, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1
-
phosphate cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.6
-
phosphate cosubstrate guanosine, pH 7.0, 25°C Mycobacterium tuberculosis
6.1
-
phosphate cosubstrate 2'-deoxyinosine, pH 7.0, 25°C Mycobacterium tuberculosis
31
-
phosphate cosubstrate 7-methylinosine, pH 7.0, 25°C Mycobacterium tuberculosis
42
-
phosphate cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C Mycobacterium tuberculosis
110
-
2'-deoxyinosine pH 7.0, 25°C Mycobacterium tuberculosis
140
-
Inosine pH 7.0, 25°C Mycobacterium tuberculosis
330
-
guanosine pH 7.0, 25°C Mycobacterium tuberculosis
340
-
2'-deoxyguanosine pH 7.0, 25°C Mycobacterium tuberculosis
550
-
7-Methylinosine pH 7.0, 25°C Mycobacterium tuberculosis
600
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
-
Mycobacterium tuberculosis
8400
-
phosphate cosubstrate inosine, pH 7.0, 25°C Mycobacterium tuberculosis