Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
guanosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.015 | - |
2'-deoxyguanosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.029 | - |
2-amino-6-mercapto-7-methylpurine ribonucleoside | - |
Mycobacterium tuberculosis | |
0.033 | - |
7-Methylinosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.04 | - |
Inosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.068 | - |
2'-deoxyinosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.427 | - |
phosphate | cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.427 | - |
phosphate | cosubstrate 7-methylinosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
0.583 | - |
phosphate | cosubstrate inosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
1.131 | - |
phosphate | cosubstrate 2'-deoxyinosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
1.345 | - |
phosphate | cosubstrate guanosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
2.349 | - |
phosphate | cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate | steady-state ordered bi bi kinetic mechanism, in which phosphate binds first followed by 2'-deoxyguanosine, and ribose 1-phosphate dissociates first followed by guanine. A general acid is essential for both catalysis and 2'-deoxyguanosine binding, and deprotonation of a group abolishes phosphate binding. Product release contributes to the rate-limiting step | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyguanosine + phosphate | - |
Mycobacterium tuberculosis | guanine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
2'-deoxyinosine + phosphate | - |
Mycobacterium tuberculosis | 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine | - |
? | |
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate | - |
Mycobacterium tuberculosis | alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine | - |
? | |
7-methylguanosine + phosphate | - |
Mycobacterium tuberculosis | 7-methylguanine + alpha-D-ribose 1-phosphate | - |
? | |
7-methylinosine + phosphate | - |
Mycobacterium tuberculosis | 7-methylhypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Mycobacterium tuberculosis | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Mycobacterium tuberculosis | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
additional information | enzyme is more specific to natural 6-oxopurine nucleosides and synthetic compounds, and does not catalyze the phosphorolysis of adenosine | Mycobacterium tuberculosis | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
phosphate | cosubstrate guanosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
4 | - |
guanosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
4.9 | - |
phosphate | cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
4.9 | - |
phosphate | cosubstrate inosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
5.1 | - |
2'-deoxyguanosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
5.4 | - |
Inosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
6.9 | - |
phosphate | cosubstrate 2'-deoxyinosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
7.5 | - |
2'-deoxyinosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
13.3 | - |
phosphate | cosubstrate 7-methylinosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
17.3 | - |
2-amino-6-mercapto-7-methylpurine ribonucleoside | - |
Mycobacterium tuberculosis | |
17.9 | - |
phosphate | cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C | Mycobacterium tuberculosis | |
18 | - |
7-Methylinosine | pH 7.0, 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
phosphate | cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
2.6 | - |
phosphate | cosubstrate guanosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
6.1 | - |
phosphate | cosubstrate 2'-deoxyinosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
31 | - |
phosphate | cosubstrate 7-methylinosine, pH 7.0, 25°C | Mycobacterium tuberculosis | |
42 | - |
phosphate | cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C | Mycobacterium tuberculosis | |
110 | - |
2'-deoxyinosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
140 | - |
Inosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
330 | - |
guanosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
340 | - |
2'-deoxyguanosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
550 | - |
7-Methylinosine | pH 7.0, 25°C | Mycobacterium tuberculosis | |
600 | - |
2-amino-6-mercapto-7-methylpurine ribonucleoside | - |
Mycobacterium tuberculosis | |
8400 | - |
phosphate | cosubstrate inosine, pH 7.0, 25°C | Mycobacterium tuberculosis |