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Literature summary for 2.4.2.1 extracted from

  • Wierzchowski, J.; Iwanska, B.; Bzowska, A.; Shugar, D.
    Interactions of calf spleen purine nucleoside phosphorylase with formycin B and its aglycone - spectroscopic and kinetic studies (2007), Nucleosides Nucleotides Nucleic Acids, 26, 849-854.
    View publication on PubMed
Search for more literature for 2.4.2.1

Inhibitors

Inhibitors Comment Organism Structure
7-ketopyrazolo[4,3-d]pyrimidine inhibits phosphorolysis of 7-methylguanosine uncompetitively, inhibits synthesis of 8-azaguanosine competitively Bos taurus
7KPP non-substrate inhibitor of both the phosphorolytic and reverse synthetic pathways, inhibits synthesis of 8-azaguanosine from 8-azaguanine competitively Bos taurus
formycin B inhibits phosphorolysis of 7-methylguanosine uncompetitively; weak, uncompetitive inhibitor. Formycin B forms a weakly fluorescent complex with the enzyme Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Bos taurus
-
 calf
-

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methylguanosine + phosphate
-
 Bos taurus 7-methylguanine + alpha-D-ribose 1-phosphate
-
 ?
8-azaguanine + alpha-D-ribose 1-phosphate
-
 Bos taurus 8-azaguanosine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PNP
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 7.3, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM Bos taurus
0.0028 0.0035 7-ketopyrazolo[4,3-d]pyrimidine pH 6.5, concentration of the substrate 8-azaguanosine is 0.05-0.25 mM Bos taurus
0.0036
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 7.9, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM Bos taurus
0.0037
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 8.2, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM Bos taurus
0.0042
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 8.2, concentration of the substrate 8-azaguanosine is 0.005-0.05 mM Bos taurus
0.038
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 6.0, concentration of the substrate 7-methylguanosine is 0.032 mM Bos taurus
0.054
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 7.0, concentration of the substrate 7-methylguanosine is 0.03 mM Bos taurus
0.073
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 7.0, concentration of the substrate 7-methylguanosine is 0.122 mM Bos taurus
0.093
-
 7-ketopyrazolo[4,3-d]pyrimidine pH 8.0, concentration of the substrate 7-methylguanosine is 0.032 mM Bos taurus
0.1
-
 formycin B inhibition of phosphorolysis of 7-methylguanosine Bos taurus
0.1
-
 formycin B phosphorolysis of 7-methylguanosine Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.002 0.004 synthesis of 8-azaguanosine from 8-azaguanine Bos taurus 7KPP
0.035 0.1 phosphorolysis of 7-methylguanosine Bos taurus 7KPP
0.098
-
 pH 7.0, concentration of the substrate 7-methylguanosine is 0.03 mM Bos taurus 7-ketopyrazolo[4,3-d]pyrimidine
0.098
-
 pH 7.0, concentration of the substrate 7-methylguanosine is 0.122 mM Bos taurus 7-ketopyrazolo[4,3-d]pyrimidine