Crystallization (Comment) | Organism |
---|---|
HsPNP/4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H/SO4 crystal complexes are prepared by hanging-drop vapor diffusion, HsPNP/immucillin-H/PO4 crystal complexes are prepared by sitting-drop vapor diffusion | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H257D | kcat/Km for inosine is 1460fold lower than wild-type value, greatly decreased affinity for Immucillin-H | Homo sapiens |
H257F | kcat/Km for inosine is 68fold lower than wild-type value, greatly decreased affinity for Immucillin-H | Homo sapiens |
H257G | kcat/Km for inosine is 610fold lower than wild-type value, greatly decreased affinity for Immucillin-H | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | - |
Homo sapiens | |
5'-deoxy-immucillin-H | - |
Homo sapiens | |
immucillin-H | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
Inosine | 25°C, pH 7.4 | Homo sapiens | |
0.75 | - |
Inosine | 25°C, pH 7.4 | Homo sapiens | |
1.35 | - |
Inosine | 25°C, pH 7.4 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00491 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HsPNP | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Inosine | 25°C, pH 7.4 | Homo sapiens | |
1.75 | - |
Inosine | 25°C, pH 7.4 | Homo sapiens | |
6.55 | - |
Inosine | 25°C, pH 7.4 | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000000077 | - |
5'-deoxy-immucillin-H | 25°C, pH 7.4, mutant enzyme H257G | Homo sapiens | |
0.0000000107 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | 25°C, pH 7.4, wild-type enzyme, measured in slow-onset tight-binding phase | Homo sapiens | |
0.000000011 | - |
immucillin-H | 25°C, pH 7.4, mutant enzyme H257G | Homo sapiens | |
0.00000002 | - |
5'-deoxy-immucillin-H | 25°C, pH 7.4, wild-type enzyme | Homo sapiens | |
0.000000054 | - |
5'-deoxy-immucillin-H | 25°C, pH 7.4, mutant enzyme H257D | Homo sapiens | |
0.0000000579 | - |
immucillin-H | 25°C, pH 7.4, wild-type enzyme, measured in slow-onset tight-binding phase | Homo sapiens | |
0.000000086 | - |
immucillin-H | 25°C, pH 7.4, mutant enzyme H257D | Homo sapiens | |
0.000000172 | - |
immucillin-H | 25°C, pH 7.4, mutant enzyme H257F | Homo sapiens | |
0.000000198 | - |
5'-deoxy-immucillin-H | 25°C, pH 7.4, mutant enzyme H257F | Homo sapiens | |
0.00000027 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | 25°C, pH 7.4, mutant enzyme H257G, measured in slow-onset tight-binding phase | Homo sapiens | |
0.0000009 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | 25°C, pH 7.4, mutant enzyme H257D, measured in slow-onset tight-binding phase | Homo sapiens | |
0.00000095 | - |
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | 25°C, pH 7.4, mutant enzyme H257F, measured in slow-onset tight-binding phase | Homo sapiens |