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Literature summary for 2.4.2.1 extracted from

  • Wielgus-Kutrowska, B.; Bzowska, A.; Tebbe, J.; Koeller, G.; Shugar, D.
    Purine nucleoside phosphorylase from Cellulomonas sp.: physicochemical properties and binding of substrates determined by ligand-dependent enhancement of enzyme intrinsic fluorescence, and by protective effects of ligands on thermal inactivation of the enzyme (2002), Biochim. Biophys. Acta, 1597, 320-334.
    View publication on PubMed
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29035
-
 3 * 29035, mass spectrometry Cellulomonas sp.
114000
-
 gel filtration Cellulomonas sp.

Organism

Organism UniProt Comment Textmining
Cellulomonas sp.
-
-
-

Subunits

Subunits Comment Organism
trimer 3 * 29035, mass spectrometry Cellulomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.4, 10 min, about 10% loss of activity Cellulomonas sp.
60
-
 pH 7.4, 10 min, about 10% loss of activity Cellulomonas sp.
62
-
 pH 7.4, 50% inactivation Cellulomonas sp.
65
-
 pH 7.4, 10 min, about 75% loss of activity Cellulomonas sp.
70
-
 denaturation above Cellulomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Cellulomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 65% of maximal activity, pH 9.0: about 35% of maximal activity Cellulomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 37°C, 10 min, 75% loss of activity Cellulomonas sp.
6
-
 37°C, 10 min, 65% loss of activity Cellulomonas sp.
7
-
 37°C, 10 min, 50% loss of activity Cellulomonas sp.
8
-
 37°C, 10 min, about 30% loss of maximal activity Cellulomonas sp.
9
-
 37°C, 10 min, 20% loss of activity Cellulomonas sp.
10
-
 37°C, 10 min, 10% loss of activity Cellulomonas sp.