Crystallization (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | - |
Escherichia coli | |
PCMB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
alpha-D-ribose 1-phosphate | reaction with adenine | Escherichia coli | |
0.07 | - |
Inosine | - |
Escherichia coli | |
0.09 | - |
Inosine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
phosphate | reaction with inosine | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
deoxyribose 1-phosphate | reaction with adenine | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
alpha-D-deoxyribose 1-phosphate | reaction with adenine | Escherichia coli | |
0.12 | - |
phosphate | reaction with inosine | Escherichia coli | |
0.17 | - |
deoxyinosine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.18 | - |
deoxyinosine | - |
Escherichia coli | |
0.63 | - |
arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.8 | - |
arsenate | reaction with inosine | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23700 | - |
6 * 23700, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
23700 | - |
6 * 23700, SDS-PAGE | Escherichia coli |
138000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
138000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
195 | - |
- |
Escherichia coli |
301 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4°C, stable for 2 years | Salmonella enterica subsp. enterica serovar Typhimurium |
4°C, stable for 2 years | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + deoxyribose 1-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | deoxyadenosine + phosphate | - |
? | |
adenosine + arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenine + alpha-D-ribose 1-arsenate | - |
? | |
adenosine + arsenate | - |
Escherichia coli | adenine + alpha-D-ribose 1-arsenate | - |
? | |
adenosine + phosphate | 78% of the activity with deoxyguanosine | Salmonella enterica subsp. enterica serovar Typhimurium | adenine + alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | 61% of the activity with deoxyguanosine | Escherichia coli | adenine + alpha-D-ribose 1-phosphate | - |
? | |
deoxyadenosine + phosphate | 78% of the activity with deoxyguanosine | Salmonella enterica subsp. enterica serovar Typhimurium | alpha-D-ribose 1-phosphate + adenine | - |
? | |
deoxyadenosine + phosphate | 61% of the activity with deoxyguanosine | Escherichia coli | alpha-D-ribose 1-phosphate + adenine | - |
? | |
deoxyguanosine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
deoxyguanosine + phosphate | - |
Escherichia coli | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
deoxyinosine + phosphate | 88% of the activity with deoxyguanosine | Salmonella enterica subsp. enterica serovar Typhimurium | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
deoxyinosine + phosphate | 88% of the activity with deoxyguanosine | Escherichia coli | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
guanosine + arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | guanine + alpha-D-ribose 1-arsenate | - |
? | |
guanosine + arsenate | - |
Escherichia coli | guanine + alpha-D-ribose 1-arsenate | - |
? | |
guanosine + phosphate | 81% of the activity with deoxyguanosine | Salmonella enterica subsp. enterica serovar Typhimurium | guanine + alpha-D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | 48% of the activity with deoxyguanosine | Escherichia coli | guanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | hypoxanthine + ribose 1-arsenate | - |
? | |
inosine + arsenate | - |
Escherichia coli | hypoxanthine + ribose 1-arsenate | - |
? | |
inosine + phosphate | 82% of the activity with deoxyguanosine | Salmonella enterica subsp. enterica serovar Typhimurium | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
inosine + phosphate | 46% of the activity with deoxyguanosine | Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 23700, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
hexamer | 6 * 23700, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
69 | - |
0.2 M Tris-succinate buffer, pH 7.1, half-life: 11 min. In 0.2 M potassium phosphate buffer, pH 7.1, with 2 mM deoxyadenosine, half-life: 59 min | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
synthesis of inosine from hypoxanthine | Escherichia coli |
7.1 | - |
phosphorolysis of deoxyinosine | Escherichia coli |
7.5 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
7.5 | - |
phosphorolysis of inosine | Escherichia coli |