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Literature summary for 2.4.2.1 extracted from

  • Jensen, K.F.; Nygaard, P.
    Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhimurium. Purification and some properties (1975), Eur. J. Biochem., 51, 253-265.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
PCMB
-
Escherichia coli
PCMB
-
Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
alpha-D-ribose 1-phosphate reaction with adenine Escherichia coli
0.07
-
Inosine
-
Escherichia coli
0.09
-
Inosine
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
phosphate reaction with inosine Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
deoxyribose 1-phosphate reaction with adenine Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
alpha-D-deoxyribose 1-phosphate reaction with adenine Escherichia coli
0.12
-
phosphate reaction with inosine Escherichia coli
0.17
-
deoxyinosine
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.18
-
deoxyinosine
-
Escherichia coli
0.63
-
arsenate
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.8
-
arsenate reaction with inosine Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23700
-
6 * 23700, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
23700
-
6 * 23700, SDS-PAGE Escherichia coli
138000
-
gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
138000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
195
-
-
Escherichia coli
301
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, stable for 2 years Salmonella enterica subsp. enterica serovar Typhimurium
4°C, stable for 2 years Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + deoxyribose 1-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium deoxyadenosine + phosphate
-
?
adenosine + arsenate
-
Salmonella enterica subsp. enterica serovar Typhimurium adenine + alpha-D-ribose 1-arsenate
-
?
adenosine + arsenate
-
Escherichia coli adenine + alpha-D-ribose 1-arsenate
-
?
adenosine + phosphate 78% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium adenine + alpha-D-ribose 1-phosphate
-
?
adenosine + phosphate 61% of the activity with deoxyguanosine Escherichia coli adenine + alpha-D-ribose 1-phosphate
-
?
deoxyadenosine + phosphate 78% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium alpha-D-ribose 1-phosphate + adenine
-
?
deoxyadenosine + phosphate 61% of the activity with deoxyguanosine Escherichia coli alpha-D-ribose 1-phosphate + adenine
-
?
deoxyguanosine + phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium guanine + alpha-D-deoxyribose 1-phosphate
-
?
deoxyguanosine + phosphate
-
Escherichia coli guanine + alpha-D-deoxyribose 1-phosphate
-
?
deoxyinosine + phosphate 88% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
deoxyinosine + phosphate 88% of the activity with deoxyguanosine Escherichia coli hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
guanosine + arsenate
-
Salmonella enterica subsp. enterica serovar Typhimurium guanine + alpha-D-ribose 1-arsenate
-
?
guanosine + arsenate
-
Escherichia coli guanine + alpha-D-ribose 1-arsenate
-
?
guanosine + phosphate 81% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium guanine + alpha-D-ribose 1-phosphate
-
?
guanosine + phosphate 48% of the activity with deoxyguanosine Escherichia coli guanine + alpha-D-ribose 1-phosphate
-
?
inosine + arsenate
-
Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + ribose 1-arsenate
-
?
inosine + arsenate
-
Escherichia coli hypoxanthine + ribose 1-arsenate
-
?
inosine + phosphate 82% of the activity with deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium hypoxanthine + alpha-D-ribose 1-phosphate
-
r
inosine + phosphate 46% of the activity with deoxyguanosine Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 23700, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
hexamer 6 * 23700, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
69
-
0.2 M Tris-succinate buffer, pH 7.1, half-life: 11 min. In 0.2 M potassium phosphate buffer, pH 7.1, with 2 mM deoxyadenosine, half-life: 59 min Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
synthesis of inosine from hypoxanthine Escherichia coli
7.1
-
phosphorolysis of deoxyinosine Escherichia coli
7.5
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
7.5
-
phosphorolysis of inosine Escherichia coli