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Literature summary for 2.4.2.1 extracted from
- Purine nucleoside phosphorylase from rabbit liver (1978), Methods Enzymol., 51, 524-530.
General Stability
| General Stability | Organism |
|---|---|
| stabilized by 2-mercaptoethanol during the purification | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-ribose 1-phosphate | product inhibition | Oryctolagus cuniculus |  |
| guanine | product inhibition | Oryctolagus cuniculus | |
| hypoxanthine | product inhibition | Oryctolagus cuniculus | |
| PCMB | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Inosine | - |
Oryctolagus cuniculus | |
| 0.05 | - |
guanosine | - |
Oryctolagus cuniculus | |
| 0.1 | - |
deoxyguanosine | - |
Oryctolagus cuniculus | |
| 0.133 | - |
Inosine | - |
Oryctolagus cuniculus | |
| 15.4 | - |
phosphate | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30500 | - |
2 * 30500, SDS-PAGE | Oryctolagus cuniculus |
| 61000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| highly susceptible to photooxidation in presence of methylene blue, maximal photoinactivation near pH 8.5 | Oryctolagus cuniculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.12 | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyguanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Oryctolagus cuniculus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30500, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Oryctolagus cuniculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | 40°C, 10 min, stable | Oryctolagus cuniculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00568 | - |
PCMB | - |
Oryctolagus cuniculus | |
| 0.0125 | - |
guanine | - |
Oryctolagus cuniculus | |
| 0.025 | - |
hypoxanthine | - |
Oryctolagus cuniculus | |
| 0.361 | - |
alpha-D-ribose 1-phosphate | - |
Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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