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Literature summary for 2.4.2.1 extracted from
- Properties of purine nucleoside phosphorylase from Enterobacter cloacae (1981), Agric. Biol. Chem., 45, 1801-1807.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0578 | - |
phosphate | - |
Enterobacter cloacae |  |
| 0.09 | - |
Inosine | - |
Enterobacter cloacae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
gel filtration | Enterobacter cloacae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alcaligenes faecalis | - |
KY3106 | - |
| Alcaligenes faecalis KY3106 | - |
KY3106 | - |
| Arthrobacter nucleogenes | - |
KY3168 | - |
| Arthrobacter nucleogenes KY3168 | - |
KY3168 | - |
| Bacterium cadaveris | - |
KY3402 | - |
| Bacterium cadaveris KY3402 | - |
KY3402 | - |
| Cellulomonas cellasea | - |
KY3491 | - |
| Cellulomonas cellasea KY3491 | - |
KY3491 | - |
| Corynebacterium vitaeruminis | - |
KY3459 | - |
| Corynebacterium vitaeruminis KY3459 | - |
KY3459 | - |
| Enterobacter cloacae | - |
KY3074 | - |
| Enterobacter cloacae KY3074 | - |
KY3074 | - |
| Micrococcus luteus | - |
KY 3760 | - |
| Micrococcus luteus KY 3760 | - |
KY 3760 | - |
| Proteus mirabilis | - |
KY4057 | - |
| Proteus mirabilis KY4057 | - |
KY4057 | - |
| Xanthomonas campestris | - |
KY4208 | - |
| Xanthomonas campestris KY4208 | - |
KY4208 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Enterobacter cloacae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Enterobacter cloacae |
| 7.9 | - |
- |
Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyadenosine + phosphate | 112.9% of the activity with inosine | Enterobacter cloacae | alpha-D-ribose 1-phosphate + adenine | - |
? | |
| deoxyadenosine + phosphate | 112.9% of the activity with inosine | Enterobacter cloacae KY3074 | alpha-D-ribose 1-phosphate + adenine | - |
? | |
| guanosine + phosphate | 47.4% of the reaction with inosine | Enterobacter cloacae | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | 47.4% of the reaction with inosine | Enterobacter cloacae KY3074 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | the equilibrium is reached when approximately 14% of inosine is phosphorylated | Enterobacter cloacae | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | the equilibrium is reached when approximately 14% of inosine is phosphorylated | Enterobacter cloacae KY3074 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| xanthosine + phosphate | 43.5% of the activity with inosine | Enterobacter cloacae | alpha-D-ribose 1-phosphate + xanthine | - |
? | |
| xanthosine + phosphate | 43.5% of the activity with inosine | Enterobacter cloacae KY3074 | alpha-D-ribose 1-phosphate + xanthine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Enterobacter cloacae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 75% of maximal activity, 70°C: about 80% of maximal activity | Enterobacter cloacae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, stable | Enterobacter cloacae |
| 70 | - |
30 min, about 90% loss of activity | Enterobacter cloacae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Enterobacter cloacae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6.0: about 60% of maximal activity, pH 10.0: about 75% of maximal activity | Enterobacter cloacae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
80°C, 30 min, about 75% loss of activity | Enterobacter cloacae |
| 7 | 7.3 | 80°C, 30 min, stable | Enterobacter cloacae |
| 8 | - |
80°C, 30 min, about 35% loss of activity | Enterobacter cloacae |
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