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Literature summary for 2.4.2.1 extracted from

  • Utagawa, T.; Morisawa, H.; Yamanaka, S.; Yamazaki, A.; Yoshinaga, F.; Hirose, Y.
    Properties of nucleoside phosphorylase from Enterobacter aerogenes (1985), Agric. Biol. Chem., 49, 3239-3246.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
Inosine 40°C Klebsiella aerogenes
2.2
-
Inosine 60°C Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
30°C, 1 month, less than 20% loss of activity Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine arabinoside + phosphate 49% of the activity with adenosine Klebsiella aerogenes alpha-D-arabinose 1-phosphate + adenine
-
?
adenosine + phosphate best substrate Klebsiella aerogenes adenine + alpha-D-ribose 1-phosphate
-
?
adenosine + phosphate best substrate Klebsiella aerogenes AJ 11125 adenine + alpha-D-ribose 1-phosphate
-
?
deoxyadenosine + phosphate 2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine Klebsiella aerogenes alpha-D-ribose 1-phosphate + adenine
-
?
deoxyadenosine + phosphate 2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine Klebsiella aerogenes AJ 11125 alpha-D-ribose 1-phosphate + adenine
-
?
deoxyinosine + phosphate 2'-deoxyinosine, 43% of the activity with adenosine Klebsiella aerogenes hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
deoxyinosine + phosphate 2'-deoxyinosine, 43% of the activity with adenosine Klebsiella aerogenes AJ 11125 hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
inosine + phosphate 34% of the activity with adenosine Klebsiella aerogenes hypoxanthine + alpha-D-ribose 1-phosphate
-
r
inosine + phosphate equilibrium when 65% of inosine has been phosphorylated Klebsiella aerogenes hypoxanthine + alpha-D-ribose 1-phosphate
-
r
inosine + phosphate 34% of the activity with adenosine Klebsiella aerogenes AJ 11125 hypoxanthine + alpha-D-ribose 1-phosphate
-
r
inosine + phosphate equilibrium when 65% of inosine has been phosphorylated Klebsiella aerogenes AJ 11125 hypoxanthine + alpha-D-ribose 1-phosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Klebsiella aerogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
1 month, less than 20% loss of activity Klebsiella aerogenes
60
-
half-life: around 1 week Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Klebsiella aerogenes