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Literature summary for 2.4.2.1 extracted from
- Human erythrocyte purine nucleoside phosphorylase: molecular weight and physical properties. A Theorell-Chance catalytic mechanism (1979), J. Biol. Chem., 254, 9927-9932.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-ribose 1-phosphate | - |
Homo sapiens |  |
| guanine | - |
Homo sapiens | |
| guanosine | product inhibition | Homo sapiens | |
| phosphate | product inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0308 | - |
guanine | - |
Homo sapiens | |
| 0.039 | - |
hypoxanthine | - |
Homo sapiens | |
| 0.0443 | - |
guanosine | - |
Homo sapiens | |
| 0.0443 | - |
deoxyguanosine | - |
Homo sapiens | |
| 0.05 | - |
Inosine | - |
Homo sapiens | |
| 0.167 | - |
6-mercaptoguanosine | - |
Homo sapiens | |
| 0.228 | - |
alpha-D-ribose 1-phosphate | reaction with guanine | Homo sapiens | |
| 13.1 | - |
phosphate | reaction with guanosine | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Homo sapiens |
| 67000 | - |
equilibrium sedimentation, gel filtration, amino acid analysis | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate | Theorell-Chance mechanism | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 55 | - |
reaction with guanosine | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-mercaptoguanosine + phosphate | - |
Homo sapiens | alpha-D-ribose 1-phosphate + 6-mercaptoguanine | - |
? | |
| deoxyguanosine + phosphate | - |
Homo sapiens | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Homo sapiens | guanine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 2 * 32000, SDS-PAGE | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
guanosine | with guanine as variable substrate and ribose 1-phosphate as fixed substrate | Homo sapiens | |
| 0.0125 | - |
guanine | with guanosine as variable substrate and phosphate as fixed substrate | Homo sapiens | |
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