Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | - |
Leuconostoc mesenteroides | |
phosphate | - |
Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + (R,S)-1,2-butandiol | regioselective glucosylation | Leuconostoc mesenteroides | phosphate + 2-O-(alpha-D-glucopyranosyl)-1,2-butandiol | - |
? | |
alpha-D-glucose 1-phosphate + glycerol | The glucoside yield is higher when sucrose is used as a donor rather than alpha-D-glucose 1-phosphate, due to the fact that the released phosphate is a stronger inhibitor of the enzyme in case of alpha-D-glucose 1-phosphate than the released fructose in case of sucrose | Leuconostoc mesenteroides | phosphate + 2-O-alpha-D-glucopyranosyl-sn-glycerol | - |
? | |
additional information | regio- and stereoselective glucosylation of diols by sucrose phosphorylase using sucrose or glucose 1-phosphate as glucosyl donor, stereochemistry of products from glucosyl transfer and phosphorolysis an hydrolysis reactions, NMR analysis, overview. Mono-alcohols are not accepted as substrates but several 1,2-diols are readily glucosylated, proving that the vicinal diol unit is crucial for activity. The smallest substrate that is accepted for glucosylation appears to be ethylene glycol, it is converted to the monoglucoside by 69%. No activity with (R,S)-3-amino-1,2-propanediol (R,S)-3-chloro-1,2-propanediol, (R,S)-1-thioglycerol, and (R,S)-glyceraldehyde | Leuconostoc mesenteroides | ? | - |
? | |
sucrose + (R)-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + (R,S)-1,2-butandiol | regioselective glucosylation, sucrose is the preferred glucosyl donor with 1,2-butandiol compared to alpha-D-glucose 1-phosphate | Leuconostoc mesenteroides | D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-butandiol | - |
? | |
sucrose + (R,S)-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + (R,S)-3-methoxy-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 3-methoxy-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + (S)-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + 3-tert-butoxy-1,2-propanediol | regioselective glucosylation | Leuconostoc mesenteroides | D-fructose + 3-tert-butoxy-2-O-(alpha-D-glucopyranosyl)-1,2-propanediol | - |
? | |
sucrose + ethylene glycol | - |
Leuconostoc mesenteroides | D-fructose + 2-O-alpha-D-glucopyranosyl-ethylene glycol | - |
? | |
sucrose + glycerol | regio- and stereoselective glucosylation. The glucoside yield is higher when sucrose is used as a donor rather than alpha-D-glucose 1-phosphate, due to the fact that the released phosphate is a stronger inhibitor of the enzyme in case of alpha-D-glucose 1-phosphate than the released fructose in case of sucrose | Leuconostoc mesenteroides | D-fructose + 2-O-alpha-D-glucopyranosyl-sn-glycerol | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
assay at | Leuconostoc mesenteroides |