Cloned (Comment) | Organism |
---|---|
gene 1355SPase, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain DH5alpha | Leuconostoc mesenteroides |
Protein Variants | Comment | Organism |
---|---|---|
D295E | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Leuconostoc mesenteroides |
D295N | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56600 | - |
x * 56600, about, sequence calculation, x * 60600, recombinant His-tagged enzyme, SDS-PAGE | Leuconostoc mesenteroides |
60600 | - |
x * 56600, about, sequence calculation, x * 60600, recombinant His-tagged enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + D-glucose 1-phosphate | - |
? | |
sucrose + phosphate | Leuconostoc mesenteroides NRRL B-1355 | - |
D-fructose + D-glucose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | B0F411 | gene 1355SPase | - |
Leuconostoc mesenteroides NRRL B-1355 | B0F411 | gene 1355SPase | - |
Purification (Comment) | Organism |
---|---|
reconbinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography | Leuconostoc mesenteroides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | constitutive enzyme | Leuconostoc mesenteroides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
purified enzyme, substrate sucrose | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows high substrate specificity towards glucosyl donors accepting only sucrose, glucose 1-phosphate, and glucose 1-fluoride, but a broad substrate specificity towards glycosyl acceptors, overview. No activity with melibiose, melezitose, and raffinose | Leuconostoc mesenteroides | ? | - |
? | |
additional information | the enzyme shows high substrate specificity towards glucosyl donors accepting only sucrose, glucose 1-phosphate, and glucose 1-fluoride, but a broad substrate specificity towards glycosyl acceptors, overview. No activity with melibiose, melezitose, and raffinose | Leuconostoc mesenteroides NRRL B-1355 | ? | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + D-glucose 1-phosphate | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides NRRL B-1355 | D-fructose + D-glucose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56600, about, sequence calculation, x * 60600, recombinant His-tagged enzyme, SDS-PAGE | Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
1355SPase | - |
Leuconostoc mesenteroides |
SPase | - |
Leuconostoc mesenteroides |
sucrose: orthophosphate, alpha-D-glucosyltransferase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Leuconostoc mesenteroides |