Cloned (Comment) | Organism |
---|---|
in pKN55-N6His-TEV for expression in Pichia pastoris strain SMD1168 and with TEV-cleavable hexa-His-tag | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
hT10 | GalNAc-T10, lacking transmembrane domain (AA1-70) | Homo sapiens |
hT10CD | GalNAc-T10 catalytic domain, hT10 lacking lectin domain (AA446-603) and transmembrane domain (AA1-70), no altered preference in glycosylation site selection, loss of initial burst phase during glycosylation of MUC5AC-13 at Thr-9, gain of initial burst phase during glycosylation of MUC5AC-3 at Thr-9, GalNAc-transfer accompanied by high rate of UDP-GalNAc hydrolysis, lectin domain not required for catalysis, no glycosylation of MUC5AC-9 | Homo sapiens |
hT10CD-hT2LD | GalNAc-T10 catalytic domain-GalNAc-T2 lectin domain, hT10 lacking lectin domain (AA446-603) and transmembrane domain (AA1-70) fused to hT2 lectin domain, no glycosylation of MUC5AC-9 | Homo sapiens |
hT2 | GalNAc-T2, lacking transmembrane domain (AA1-74) | Homo sapiens |
hT2CD | GalNAc-T2 catalytic domain, hT2 lacking lectin domain (AA441-571) and transmembrane domain (AA1-74), reduced affinity for substrate MUC5AC-13 but not for MUC5AC-3, shift of preferred MUC5AC-3 glycosylation site from Thr-9 to Thr-13 | Homo sapiens |
hT2CD-hT10LD | GalNAc-T2 catalytic domain-GalNAc-T10 lectin domain, hT2 lacking lectin domain (AA441-571) and transmembrane domain (AA1-74) fused to hT10 lectin domain, partially restored glycosylation to Thr-3 on MUC5AC-13 but not to Thr-13 on MUC5AC-3 or Ser-5 on MUC5AC-3,13 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
MUC5AC-13 | at site Thr-3, hT2 | Homo sapiens | |
0.018 | - |
MUC5AC | at site Thr-9, hT2CD | Homo sapiens | |
0.02 | - |
MUC5AC | at site Thr-9, hT2 | Homo sapiens | |
0.033 | - |
MUC5AC-3 | at site Thr-13, hT2 | Homo sapiens | |
0.036 | - |
MUC5AC-3 | at site Thr-9, hT2CD | Homo sapiens | |
0.05 | - |
MUC5AC-3 | at site Thr-2, hT10CD | Homo sapiens | |
0.096 | - |
MUC5AC-3 | at site Thr-2, hT10 | Homo sapiens | |
0.107 | - |
MUC5AC-3 | at site Thr-2, hT10CD-hT2LD | Homo sapiens | |
0.17 | - |
MUC5AC-13 | at site Thr-9, hT2CD | Homo sapiens | |
0.44 | - |
MUC5AC-3,13 | at sites Thr-2 and Thr12, hT10 | Homo sapiens | |
0.53 | - |
MUC5AC-13 | at site Thr-12, hT10 | Homo sapiens | |
0.57 | - |
MUC5AC-3,13 | at sites Thr-2 and Thr12, hT10CD-hT2LD | Homo sapiens | |
0.64 | - |
MUC5AC-13 | at site Thr-12, hT10CD-hT2LD | Homo sapiens | |
0.77 | - |
MUC5AC-13 | at site Thr-12, hT10CD | Homo sapiens | |
1 | - |
MUC5AC-3,13 | at sites Thr-2 and Thr12, hT10CD | Homo sapiens | |
2.4 | - |
MUC5AC-9 | at site Thr-2, hT10 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | mucin-type O-linked glycosylation of serine or threonine residues | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoforms ppGalNAcT-2 (hT2) and ppGalNAcT-10 (hT10) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | ppGalNAcT-10 amplified from isolated total RNA | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
MUC5AC + UDP-GalNAc | hT2CD, hT2: glycosylation site selection driven by and substrate binding through its catalytic domain, hT10: not catalysed by hT10 because hT10 does not recognize naked peptides | Homo sapiens | GalNAc-Thr9-MUC5AC + UDP | preferred site for glycosylation by hT2 and hT2CD compared to Thr-3, Thr-10 and Thr-13 | ? | |
MUC5AC glycopeptides + UDP-GalNAc | hT2: site preference of glycosylation depends on location of pre-existing GalNAc and is mediated by its lectin domain which directs glycosylation 10 residues N- or C-terminal to an extant GalNAc residue and aids site selection only when potential site is N-terminal to an extant GalNAc residue not C-terminal, Thr-9 is no preferred site of glycosylation, hT10: must recognize existing GalNAc residues via its catalytic domain which directs selection to the glycosylation site immediately N-terminal to the present GalNAc, | Homo sapiens | GalNAc-MUC5AC + UDP | - |
? | |
MUC5AC-13 + UDP-GalNAc | hT10, hT10CD, hT10CD-hT2LD | Homo sapiens | GalNAc-Thr12-MUC5AC-13 + UDP | - |
? | |
MUC5AC-13 + UDP-GalNAc | hT2: substrate binding through its catalytic domain, hT10: transfer of only single GalNAc residues | Homo sapiens | GalNAc-Thr3-MUC5AC-13 + UDP | predominant product of hT2 and hT10 | ? | |
MUC5AC-13 + UDP-GalNAc | hT2, hT2CD: no initial burst phase during catalysis | Homo sapiens | GalNAc-Thr9-MUC5AC-13 + UDP | not preferred by hT2, preferred by hT2CD | ? | |
MUC5AC-3 + UDP-GalNAc | hT2: no initial burst phase during catalysis, substrate binding through its catalytic domain, hT10: transfer of only single GalNAc residues | Homo sapiens | GalNAc-Thr13-MUC5AC-3 + UDP | hT2: preferred site for glycosylation, mediated by enhanced product release through lectin domain, hT10: predominant product | ? | |
MUC5AC-3 + UDP-GalNAc | hT10CD, hT10CD-hT2LD, hT10: catalysis faster than for MUCAC-9 | Homo sapiens | GalNAc-Thr2-MUC5AC + UDP | - |
? | |
MUC5AC-3 + UDP-GalNAc | hT2, hT2CD | Homo sapiens | GalNAc-Thr9-MUC5AC-3 + UDP | not preferred by hT2, hT2CD: preferred site for glycosylation, lack of lectin domain in hT2CD results in rate-limiting product release | ? | |
MUC5AC-3,13 + UDP-GalNAc | hT2, not catalysed by hT2CD and hT2CD-hT10LD | Homo sapiens | GalNAc-Ser-5-MUC5AC-3,13 + UDP | preferred site by hT2 | ? | |
MUC5AC-3,13 + UDP-GalNAc | hT10, hT10CD, hT10CD-hT2LD | Homo sapiens | GalNAc-Thr2,12-MUC5AC-3,13 + UDP | - |
? | |
MUC5AC-9 + UDP-GalNAc | not catalysed by hT10CD and hT10CD-hT2LD, hT10: catalysis slower than for MUC5AC-3 | Homo sapiens | GalNAc-Thr2-MUC5AC-9 + UDP | preferred site of glycosylation by hT10 | ? | |
UDP-N-acetyl-D-galactosamine + polypeptide | mucin-type O-linked glycosylation of serine or threonine residues | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ppGalNAcT or hT | - |
Homo sapiens |
UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | hT2 catalysed glycosylation of MUC5AC and MUC5AC-13 follows a biphasic time course: fast formation of enzyme-product (initial burst phase: 1 molecule product per molecule hT2 enzyme), slow and rate-limiting product release | Homo sapiens | |
additional information | - |
additional information | hT2 catalysed glycosylation of MUC5AC-3 follows a linear time course: rate limiting catalysis or substrate binding | Homo sapiens | |
additional information | - |
additional information | steady-state rates of hT2 catalysis are less than 1 reaction per second at room temperature | Homo sapiens | |
0.04 | - |
MUC5AC | at site Thr-9, hT2, at pre-steady state | Homo sapiens | |
0.04 | - |
MUC5AC-3 | at site Thr-9, hT2CD, at pre-steady state | Homo sapiens | |
0.05 | - |
MUC5AC | at site Thr-9, hT2CD | Homo sapiens | |
0.052 | - |
MUC5AC | at site Thr-9, hT2 | Homo sapiens | |
0.053 | - |
MUC5AC | at site Thr-9, hT2CD, at pre-steady state | Homo sapiens | |
0.058 | - |
MUC5AC-3 | at site Thr-9, hT2CD | Homo sapiens | |
0.067 | - |
MUC5AC-13 | at site Thr-9, hT2CD | Homo sapiens | |
0.075 | - |
MUC5AC-13 | at site Thr-9, hT2CD, at pre-steady state | Homo sapiens | |
0.103 | - |
MUC5AC-13 | at site Thr-3, hT2 | Homo sapiens | |
0.108 | - |
MUC5AC-13 | at site Thr-3, hT2, at pre-steady state | Homo sapiens | |
0.123 | - |
MUC5AC | at site Thr-9, hT2, single turnover event | Homo sapiens | |
0.127 | - |
MUC5AC-9 | at site Thr-2, hT10 | Homo sapiens | |
0.137 | - |
MUC5AC-3 | at site Thr-9, hT2CD, single turnover event | Homo sapiens | |
0.15 | - |
MUC5AC-3 | at site Thr-2, hT10CD-hT2LD | Homo sapiens | |
0.17 | - |
MUC5AC-13 | at site Thr-3, hT2, single turnover event | Homo sapiens | |
0.193 | - |
MUC5AC | at site Thr-9, hT2CD, single turnover event | Homo sapiens | |
0.218 | - |
MUC5AC-3 | at site Thr-13, hT2 | Homo sapiens | |
0.22 | - |
MUC5AC-3 | at site Thr-13, hT2, at pre-steady state | Homo sapiens | |
0.24 | - |
MUC5AC-3 | at site Thr-2, hT10CD | Homo sapiens | |
0.29 | - |
MUC5AC-13 | at site Thr-12, hT10CD-hT2LD | Homo sapiens | |
0.31 | - |
MUC5AC-13 | at site Thr-12, hT10CD | Homo sapiens | |
0.33 | - |
MUC5AC-3 | at site Thr-2, hT10 | Homo sapiens | |
0.4 | - |
MUC5AC-13 | at site Thr-12, hT10 | Homo sapiens | |
0.52 | - |
MUC5AC-3,13 | at site Thr-2 and Thr-12, hT10CD-hT2LD | Homo sapiens | |
0.76 | - |
MUC5AC-3,13 | at sites Thr-2 and Thr-12, hT10CD | Homo sapiens | |
1.4 | - |
MUC5AC-3,13 | at site Thr-2 and Thr-12, hT10 | Homo sapiens |