Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.41 extracted from

  • Duclos, S.; Da Silva, P.; Vovelle, F.; Piller, F.; Piller, V.
    Characterization of the UDP-N-acetylgalactosamine binding domain of bovine polypeptide alphaN-acetylgalactosaminyltransferase T1 (2004), Protein Eng. Des. Sel., 17, 635-646.
    View publication on PubMed
Search for more literature for 2.4.1.41

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
D209A inactive mutant enzyme Bos taurus
F124A inactive mutant enzyme Bos taurus
L189A inactive mutant enzyme Bos taurus
N126A the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.4fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 5.5fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.6fold higher than the wild-type value Bos taurus
R193A inactive mutant enzyme Bos taurus
W129A the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 4.3fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 3.5fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 2.6fold higher than the wild-type value Bos taurus
W129F the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.4fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 1.8fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value Bos taurus
W129R the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 2.1fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, wild-type enzyme Bos taurus
0.014
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129F Bos taurus
0.017
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129R Bos taurus
0.029
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129A Bos taurus
0.044
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme N126A Bos taurus
0.2
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129R Bos taurus
0.205
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129F Bos taurus
0.25
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, wild-type enzyme Bos taurus
0.41
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme N126A Bos taurus
0.65
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129A Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ in coordination both with the phosphates of UDP and the DXH motif of the enzyme Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + STPSTPSTPSTPSTP
-
 Bos taurus UDP + ?
-
 ?

Synonyms

Synonyms Comment Organism
ppGaNTase
-
 Bos taurus
UDP-GalNAc:polypeptide alphaN-acetylgalactosaminyltransferase
-
 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129A Bos taurus
0.8
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129A Bos taurus
2.43
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129F Bos taurus
2.43
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129F Bos taurus
2.53
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme N126A Bos taurus
2.53
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme N126A Bos taurus
2.79
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129R Bos taurus
2.79
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129R Bos taurus
3.47
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, wild-type enzyme Bos taurus
3.47
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, wild-type enzyme Bos taurus