Literature summary for 2.4.1.41 extracted from
Wragg, S.; Hagen, F.K.; Tabak, L.A.
Identification of essential histidine residues in UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T1 (1997), Biochem. J., 328, 193-197.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression of wild-type and mutants in COS7 cells |
Bos taurus |
Protein Variants
Protein Variants |
Comment |
Organism |
H125A |
1% of the wild-type activity |
Bos taurus |
H211A |
no detectable activity |
Bos taurus |
H341A |
6% of the wild-type activity |
Bos taurus |
H344A |
no detectable activity |
Bos taurus |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
diethyldicarbonate |
binds to His; hydroxylamine partiallly protects, UDP-GalNAc protects |
Bos taurus |
|
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
UDP-N-acetyl-D-galactosamine + polypeptide |
Bos taurus |
initial step of mucin-type O-glycosylation |
UDP + N-acetyl-D-galactosaminyl-polypeptide |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Bos taurus |
- |
isozyme T1 |
- |
Posttranslational Modification
Posttranslational Modification |
Comment |
Organism |
glycoprotein |
residues Asn-95 and Asn-552 of the recombinant enzyme are occupied by N-linked sugars in Cos7 cells |
Bos taurus |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
UDP-N-acetyl-D-galactosamine + polypeptide |
- |
Bos taurus |
UDP + N-acetyl-D-galactosaminyl-polypeptide |
- |
? |
|
UDP-N-acetyl-D-galactosamine + polypeptide |
initial step of mucin-type O-glycosylation |
Bos taurus |
UDP + N-acetyl-D-galactosaminyl-polypeptide |
- |
? |
|