Literature summary for 2.4.1.41 extracted from

Hagen, F.K.; Hazes, B.; Raffo, R.; deSa, D.; Tabak, L.A.
Structure-function analysis of the UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase. Essential residues lie in a predicted active site cleft resembling a lactose repressor fold (1999), J. Biol. Chem., 274, 6797-6803.

Cloned(Commentary)

Cloned (Comment)	Organism
ppGaNTase-T1 is cloned from kidney RNA, amino acid sequence, expression of ppGaNTases as secreted forms in COS7 cells	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
D155N	ppGaNTase-T1 mutant with wild type level of enzyme activity, expression in COS7 cells is markedly compromised	Mus musculus
D156Q	ppGaNTase-T1 mutant without enzyme activity	Mus musculus
D209A	ppGaNTase-T1 mutant without enzyme activity	Mus musculus
D209E	ppGaNTase-T1 mutant with very low enzyme activity	Mus musculus
D209N	ppGaNTase-T1 mutant without enzyme activity	Mus musculus
D310N	ppGaNTase-T1 mutant with 2% of enzyme activity	Mus musculus
D375A	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus
D375N	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus
E127Q	ppGaNTase-T1 mutant with less than 1% of enzyme activity	Mus musculus
E150Q	ppGaNTase-T1 mutant with wild type level of enzyme activity	Mus musculus
E213Q	ppGaNTase-T1 mutant with less than 1% of enzyme activity	Mus musculus
E319Q	ppGaNTase-T1 mutant without enzyme activity	Mus musculus
E322Q	ppGaNTase-T1 mutant with 1% of enzyme activity	Mus musculus
E376Q	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus
H125F	active ppGaNTase-T1 mutant, near 3fold greater activity than wild type enzyme	Mus musculus
H125Q	active ppGaNTase-T1 mutant	Mus musculus
H211D	ppGaNTase-T1 mutant without enzyme activity	Mus musculus
H341A	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus
H341K	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus
H341L	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus
H341R	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus
H341V	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus
additional information	mutations in the C-terminal 128-amino acid ricin-like lectin motif of ppGaNTase-T1 do not alter its catalytic properties	Mus musculus
N320A	ppGaNTase-T1 mutant with little effect on enzyme activity	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Mus musculus	5794	-
membrane	type II membrane protein	Mus musculus	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	requirement	Mus musculus
additional information	metal-dependent enzyme	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-N-acetyl-D-galactosamine + polypeptide	Mus musculus	initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Mus musculus
recombinant ppGaNTases expressed in COS7 cells	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	enzyme structure, GT1 motif, DXD motif, Gal/GalNAc-T motif	Mus musculus	?	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	ppGaNTase-T1: EA2 peptide acceptor, interdependence of binding of UDP-GalNAc and acceptor substrate, enzyme undergoes a conformational change upon sugar binding, equilibrium between open and closed states may be used as a regulation mechanism of activity	Mus musculus	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?
UDP-N-acetyl-D-galactosamine + polypeptide	initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function	Mus musculus	UDP + N-acetyl-D-galactosaminyl-polypeptide	-	?