Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
ppGalNAc-T13 and -T1 are localized to chromosome 2 and 18, ppGalNAc-T13 is cloned and sequenced, 556-amino acid protein | Mus musculus |
ppGalNAc-T13, -T1 and -T9 are cloned, and expressed in Sf21 or High FiveTM insect cells in a soluble form, ppGalNAc-T13: gene is localized to chromosome 2, nucleotide sequence encodes a 556-amino acid protein, ppGalNAc-T1 is localized to chromosome 18 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | ppGalNAc-T13 deficient mice with decrease in Tn antigen expression in the cerebellum | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Muc7 peptide | ppGalNac-T9 | Homo sapiens | |
0.046 | - |
Muc7 peptide | ppGalNac-T13 | Homo sapiens | |
0.054 | - |
Muc7 peptide | ppGalNac-T1 | Homo sapiens | |
0.07 | - |
Muc1a' peptide | ppGalNac-T9 | Homo sapiens | |
0.07 | - |
SDC284 peptide | derived from human syndecan-3, ppGalNac-T13 | Homo sapiens | |
0.11 | - |
Muc1a' peptide | ppGalNac-T13 | Homo sapiens | |
0.19 | - |
SDC284 peptide | derived from human syndecan-3, ppGalNac-T1 | Homo sapiens | |
0.23 | - |
Muc1a' peptide | ppGalNac-T1 | Homo sapiens | |
0.38 | - |
SDC165 peptide | derived from human syndecan-3, ppGalNac-T13 | Homo sapiens | |
0.63 | - |
SDC155 peptide | derived from human syndecan-3, ppGalNac-T13 | Homo sapiens | |
0.96 | - |
SDC284 peptide | derived from human syndecan-3, ppGalNac-T9 | Homo sapiens | |
1.33 | - |
SDC106 peptide | derived from human syndecan-3, ppGalNac-T13 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
membrane | ppGaNTase-T13: type II membrane protein with a 20-amino acid transmembrane segment and an 8-amino acid N-terminal cytoplasmic domain | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | pp-GalNAc-T13 is specifically expressed in neurons and synthesizes Tn antigen | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Mus musculus | - |
UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | ppGalNAc-T13: synthesis of O-glycan, specifically the Tn antigen in neurons | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + syndecan-3 | Homo sapiens | syndecan-3 may be a natural substrate for GalNAc-T13 | UDP + N-acetyl-D-galactosaminyl-syndecan-3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
ppGalNAc-T13, -T1 and -T9 | - |
Homo sapiens | Q8IUC8 | - |
- |
Mus musculus | - |
ppGalNAc-T13, subsequently termed ppGalNAc-T8 | - |
Purification (Comment) | Organism |
---|---|
ppGalNAc-T13, -T1 and -T9, expressed in Sf21 or High FiveTM insect cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | ppGalNAc-T13 expression only in brain, exclusively in neuronal cells | Mus musculus | - |
brain | ppGalNAc-T13 is highly and restrictively expressed in brain, fetal brain, adult cerebellum and cerebral cortex, exclusively in neuronal cells | Homo sapiens | - |
brain | pp-GalNAc-T13 is specifically expressed in neurons | Homo sapiens | - |
additional information | ppGalNAc-T13: not in astrocytes | Mus musculus | - |
additional information | tissue distribution of several ppGalNAc-transferases | Mus musculus | - |
additional information | tissue distribution of several ppGalNAc-transferases | Homo sapiens | - |
additional information | ppGalNAc-T1 is ubiquitously expressed, ppGalNAc-T13: not in glioblastoma cells and primary cultured astrocytes | Homo sapiens | - |
neuroblastoma cell | ppGalNAc-T13 is abundantly expressed in neuroblastoma cells | Homo sapiens | - |
neuron | ppGalNAc-T13 is abundantly expressed in primary cultured neurons | Homo sapiens | - |
neuron | pp-GalNAc-T13 is specifically expressed in neurons | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | multiple enzyme isoforms | Mus musculus | ? | - |
? | |
additional information | multiple enzyme isoforms | Homo sapiens | ? | - |
? | |
additional information | pp-GalNAc-T13 is specifically expressed in neurons and synthesizes Tn antigen | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + Muc7 peptide | - |
Homo sapiens | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + SDC106 peptide | - |
Homo sapiens | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + SDC155 peptide | - |
Homo sapiens | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + SDC165 peptide | - |
Homo sapiens | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + SDC284 peptide | - |
Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | - |
Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | ppGalNAc-T13: acceptors are mucin peptides, such as Muc1a', Muc5Ac and Muc7, forms a triplet Tn epitope, 3 consecutive GalNAc-Ser/Thr structures, on peptides encoded in syndecan-3, different substrate specificities of ppGalNAc-T1 and -T13 | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | ppGalNAc-T13: synthesis of O-glycan, specifically the Tn antigen in neurons | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + syndecan-3 | syndecan-3 may be a natural substrate for GalNAc-T13 | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-syndecan-3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pp-GalNAc-T13 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |