Cloned (Comment) | Organism |
---|---|
ppGalNAc-T14 and -T2 are cloned and expressed in Sf21 cells in a secreted form, 2 alternatively spliced isoforms of the ppGalNAc-T14 transcript are sequenced, major splicing form: 552-amino acid protein, ppGalNAc-T14 gene is mapped to chromosome 2p23.2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
membrane | ppGalNAc-T14: type II membrane protein | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | ppGalNAc-T14 may be involved in O-glycosylation in kidney | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | catalyzes the first step in biosynthesis of O-linked oligosaccharides in many glycoproteins | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
ppGalNAc-T14 and -T2 | - |
Purification (Comment) | Organism |
---|---|
ppGalNAc-T14 and -T2, expressed in Sf21 cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | significantly high expression of ppGalNAc-T14 | Homo sapiens | - |
additional information | ppGalNAc-T14 is ubiquitously expressed | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | multiple enzyme isoforms | Homo sapiens | ? | - |
? | |
additional information | 14 distinct isoforms different with regard to their expression and specificity, but overlaps also occur, ppGalNAc-T14 forms together with ppGalNAc-T2 a subfamily of ppGalNAc-T gene family | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide | ppGalNAc-T14 can utilize GalNAc transferred peptides as substrates | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | ppGalNAc-T14: acceptors are mucin-derived peptides such as Muc2, Muc5AC, Muc7, Muc13 | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | transfer of GalNAc to a serine or threonine residue on the acceptor protein | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | ppGalNAc-T14 may be involved in O-glycosylation in kidney | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | catalyzes the first step in biosynthesis of O-linked oligosaccharides in many glycoproteins | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |