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Literature summary for 2.4.1.41 extracted from
- Substrate specificities of three members of the human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase family, GalNAc-T1, -T2, and -T3 (1997), J. Biol. Chem., 272, 23503-23514.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GalNAc-T1, -T2 and -T3 as soluble proteins in insect Sf9 cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | values for GalNAc-T1, -T2 and -T3 for different substrate peptides | Homo sapiens | |
| 0.01 | - |
UDP-GalNAc | GalNAc-T2 | Homo sapiens |  |
| 0.027 | - |
UDP-Gal | GalNAc-T2 | Homo sapiens | |
| 0.029 | - |
UDP-GalNAc | GalNAc-T3 | Homo sapiens | |
| 0.062 | - |
UDP-GalNAc | GalNAc-T1 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | recombinant GalNAc-T1, -T2 and -T3, expressed in Sf9 cells | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | requirement | Homo sapiens | |
| Mn2+ | Mn2+ cannot be replaced by Mg2+ or Ca2+ | Homo sapiens | |
| additional information | not activated by Mg2+ or Ca2+ | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-galactosamine + fibronectin | Homo sapiens | fibronectin is a physiological substrate for GalNAc-T3 | UDP + N-acetyl-D-galactosaminyl-fibronectin | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | Homo sapiens | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
GalNAc-T1, -T2 and -T3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GalNAc-T1, -T2 and -T3, expressed in Sf9 cells | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | - |
GalNAc-T2 and -T3 | Homo sapiens |
| 0.6 | - |
GalNAc-T1 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | not: UDP-GlcNAc | Homo sapiens | ? | - |
? | |
| UDP-galactose + Muc2 peptide | only GalNAc-T2, only with the Muc2 acceptor peptide | Homo sapiens | UDP + galactosyl-Muc2 peptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + fibronectin | fibronectin is a physiological substrate for GalNAc-T3 | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-fibronectin | - |
? | |
| UDP-N-acetyl-D-galactosamine + HIV-V3 peptide | HIVIIIBgp120, only GalNAc-T3 | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide | only GalNAc-T2 | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | GalNAc-T1 and -T3: strict specificity for UDP-GalNAc, GalNAc-T2: utilizes UDP-GalNAc and UDP-Gal | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | 3 GalNAc-transferases: GalNAc-T1, -T2 and -T3 with distinct but partly overlapping specificities, dependent on the primary sequence of the acceptor substrate, acceptors: peptides based on Muc1 tandem repeat, TAP24, Muc2, Muc5AC, erythropoietin, hCG-beta, HIVIIIBgp120, ovine submaxillary mucin fragment, peptide derived from fibronectin, GalNAc-T1, -T2 and -T3 react at different rates with individual sites in the Muc1 repeat | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | Homo sapiens | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
24 h, GalNAc-T1 and -T3: 40% loss of activity, GalNAc-T2: 60% loss of activity | Homo sapiens |
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