Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.5 mM, complete inhibition | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mus musculus | |
additional information | - |
additional information | Km: 2.5 mg/ml: deglycosylated bovine submaxillary mucin | Bos taurus | |
0.008 | - |
UDP-GalNAc | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Mus musculus | 16020 | - |
membrane | membrane-bound | Bos taurus | 16020 | - |
soluble | may be a proteolytic cleavage product from a membrane-bound form | Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | partially restores activity after EDTA treatment | Bos taurus | |
Co2+ | almost as effective as Mn2+, restores activity after EDTA treatment | Bos taurus | |
Mn2+ | requirement | Mus musculus | |
Mn2+ | requirement | Bos taurus | |
Mn2+ | maximal activity at 2.5 mM, restores activity after EDTA treatment | Bos taurus | |
Mn2+ | most effective cation | Bos taurus | |
Ni2+ | partially restores activity after EDTA treatment | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
gel filtration | Bos taurus |
70000 | - |
1 * 70000, SDS-PAGE | Mus musculus |
70000 | - |
1 * 70000, SDS-PAGE | Bos taurus |
71500 | - |
gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Mus musculus | key role in O-linked glycosylation, catalyzes first step in the assembly | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | Bos taurus | key role in O-linked glycosylation, catalyzes first step in the assembly | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
colostrum | - |
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 2 N-linked oligosaccharides, on most enzyme molecules both oligosaccharides are of the complex type, but some molecules contain one complex type and one high mannose type | Bos taurus |
Purification (Comment) | Organism |
---|---|
2500fold | Mus musculus |
517000fold | Bos taurus |
partial | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BW-5147 cell | lymphoma cells | Mus musculus | - |
colostrum | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
- |
Mus musculus |
1.86 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
enzyme loses activity upon storage | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Mus musculus | ? | - |
? | |
additional information | not: UDP-Glc | Bos taurus | ? | - |
? | |
additional information | not: UDP-GlcNAc | Bos taurus | ? | - |
? | |
additional information | not: UDP-Gal | Bos taurus | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + apomucin | deglycosylated bovine submaxillary mucin | Bos taurus | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | acceptor: deglycosylated bovine submaxillary mucin | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | only UDP-GalNAc serves as sugar donor | Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | only UDP-GalNAc serves as sugar donor | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | key role in O-linked glycosylation, catalyzes first step in the assembly | Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | key role in O-linked glycosylation, catalyzes first step in the assembly | Bos taurus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Mus musculus |
monomer | 1 * 70000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.6 | - |
Bos taurus |