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Literature summary for 2.4.1.41 extracted from
- Purification and characterization of a UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase specific for glycosylation of threonine residues (1992), J. Biol. Chem., 267, 12709-12716.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | activates, especially after storage | Sus scrofa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | Mn2+ is the best activator, maximal activity at 5 mM, 20 and 100 mM result in 10% and 80% inhibition | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km: 1.01 mg/ml: ovine apomucin, 2.54 mg/ml: porcine apomucin | Sus scrofa | |
| 0.006 | - |
UDP-GalNAc | ovine apomucin | Sus scrofa | |
| 7.2 | - |
Val-Lys-Thr-Glu-Ala-Thr-Thr-Phe-Ile | - |
Sus scrofa | |
| 18.6 | - |
Val-Leu-Gly-Thr-Thr-Ala-Val | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Sus scrofa | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | less stimulation than by Mn2+ | Sus scrofa | |
| Co2+ | less stimulation than by Mn2+ | Sus scrofa | |
| Cu2+ | less stimulation than by Mn2+ | Sus scrofa | |
| Mn2+ | metal-free enzyme is inactive, Mn2+ is the best activator, maximal activity at 5 mM, half-maximal activity at 0.4 mM MnCl2, 20 and 100 mM result in 10% and 80% inhibition | Sus scrofa | |
| additional information | no reactivation of metal-free enzyme by Mg2+, Zn2+ and Ca2+ | Sus scrofa | |
| Ni2+ | less stimulation than by Mn2+ | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-galactosamine + polypeptide | Sus scrofa | first step in biosynthesis of O-linked oligosaccharides in submaxillary gland | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme contains more than one N-linked oligosaccharide | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1320fold | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| submaxillary gland | high enzyme concentration | Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Sus scrofa |
| 1.65 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20 mM imidazole-HCl, pH 7.2, 0.1% Triton X-100, 5 mM MnCl2, 60% w/v glycerol, 1 year, stable | Sus scrofa |
| 4°C, 20 mM imidazole-HCl, pH 7.2, 0.1% Triton X-100, 5 mM MnCl2, 25% w/v glycerol, 1 month, 10% loss of activity | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | not: UDP-GlcNAc | Sus scrofa | ? | - |
? | |
| additional information | not: UDP-Gal | Sus scrofa | ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + Val-Leu-Gly-Thr-Thr-Ala-Val | - |
Sus scrofa | ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + Val-Lys-Thr-Glu-Ala-Thr-Thr-Phe-Ile | - |
Sus scrofa | ? | - |
? | |
| UDP-N-acetyl-D-galactosamine + apomucin | porcine apomucin | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
| UDP-N-acetyl-D-galactosamine + apomucin | ovine apomucin | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-apomucin | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | acceptors: ovine and porcine apomucin, porcine mucin-like synthetic peptides | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | specifically transfers N-acetylgalactosamine from UDP-GalNAc to the hydroxyl group of threonine, no transferase activity towards serine-containing peptides, amino acid sequence adjacent to glycosylated Thr residues may influence the rate of glycosylation | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
| UDP-N-acetyl-D-galactosamine + polypeptide | first step in biosynthesis of O-linked oligosaccharides in submaxillary gland | Sus scrofa | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
8 h, 20 mM imidazole-HCl, pH 7.2, 0.1% Triton X-100, 5 mM MnCl2, 5% w/v glycerol, 40% loss of activity | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.2 | - |
Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | pH 4.5: no activity, pH 6.8: gradual decrease of activity below, pH 9.5: 16% of activity maximum | Sus scrofa |
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