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Literature summary for 2.4.1.41 extracted from

  • Takeuchi, M.; Yoshikawa, M.; Sasaki, R.; Chiba, R.
    Purification and characterization of UDP-N-acetylgalactosamine:kappa-casein polypeptide N-acetylgalactosaminyltransferase from mammary gland of lacting cow (1985), Agric. Biol. Chem., 49, 1059-1069.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
DTT required for maximal activity Bos taurus
Triton X-100 required for maximal activity Bos taurus

Application

Application Comment Organism
molecular biology enzyme will be useful for the in vitro glycosylation of proteins obtained from microorganisms by gene manipulation techniques Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
ADP weak Bos taurus
alphaS1-casein strong inhibition of GalNAc transfer to kappa-casein, reversed by Ca2+ Bos taurus
AMP
-
Bos taurus
ATP weak Bos taurus
CDP
-
Bos taurus
CMP
-
Bos taurus
CTP
-
Bos taurus
GDP weak Bos taurus
GMP
-
Bos taurus
GTP
-
Bos taurus
Helix pomatia lectin
-
Bos taurus
additional information not inhibited by wheat germ lectin Bos taurus
Soybean lectin
-
Bos taurus
UDP strong Bos taurus
UMP
-
Bos taurus
UTP
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km: 0.192 mg/ml: apomucin, 1.15 mg/ml: kappa-subcomponent 1, 5.1 mg/ml: kappa-subcomponent 7 Bos taurus
0.0162
-
UDP-GalNAc
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane specifically localized in Bos taurus 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ stimulates to a smaller extent than Mn2+ Bos taurus
Ca2+ stimulates to a smaller extent than Mn2+ Bos taurus
Mg2+ stimulates to a smaller extent than Mn2+ Bos taurus
Mn2+ requirement Bos taurus
Mn2+ strong stimulation, maximal activity at 4-10 mM Bos taurus
Zn2+ stimulates to a smaller extent than Mn2+ Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + kappa-casein Bos taurus glycosylation of kappa-casein occurs after casein micelle formation triggered by the accumulation of Ca2+ in vivo UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
lactating cow
-

Purification (Commentary)

Purification (Comment) Organism
7500fold Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00863
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: alphaS1-casein, alphaS2-casein, beta-casein, alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin Bos taurus ?
-
?
UDP-N-acetyl-D-galactosamine + apomucin best substrate, poorer substrates: asialo mucin, native mucin Bos taurus UDP + N-acetyl-D-galactosaminyl-apomucin
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein kappa-subcomponent 1, the nonglycosylated kappa-casein, is the best substrate among kappa-casein subcomponents, asialo kappa-casein, agalacto kappa-casein and nature kappa-casein Bos taurus UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein glycosylation of kappa-casein occurs after casein micelle formation triggered by the accumulation of Ca2+ in vivo Bos taurus UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
?
UDP-N-acetyl-D-galactosamine + polypeptide acceptor: asialo mucin, subcomponents of kappa-casein, asialo kappa-casein, agalacto kappa-casein Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?
UDP-N-acetyl-D-galactosamine + polypeptide acceptors: apomucin, kappa-casein Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 35 1 h, less than 20% loss of activity Bos taurus
45
-
1 h, 50% loss of activity Bos taurus
55
-
1 h, complete loss of activity Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 6.4 Bos taurus
6.5 7.5 Tris, imidazole and MES buffer, kappa-casein Bos taurus