Cloned (Comment) | Organism |
---|---|
expression of truncated wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant GTA/GTB mutant chimeric enzymes, complexing with synthetic antigen disaccharides or UDP, hanging drop vapour diffusion method, different solutions for the different chimeric mutants, X-ray diffraction structure determinationand analysis at 1.41-1.75 A resolution, overview, structure modelling | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of GTA/GTB chimeric enzymes GTB/G176R and GTB/G176R/G235S | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme catalyzes the final step in the enzymatic synthesis of the ABO(H) blood group A antigen, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16442 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes from Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes the final step in the enzymatic synthesis of the ABO(H) blood group A antigen, overview | Homo sapiens | ? | - |
? | |
additional information | substrate binding structure by wild-type and mutant GTA, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis of GTA/GTB chimeric enzymes GTB/G176R and GTB/G176R/G235S bound to a panel of donor and acceptor analogue substrates, open, semi-closed, and closed conformations, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ABO(H) blood group A glycosyltransferase | - |
Homo sapiens |
alpha-(1,3)-N-acetylgalactosaminyltransferase | - |
Homo sapiens |
GTA | - |
Homo sapiens |