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Literature summary for 2.4.1.40 extracted from

  • Alfaro, J.A.; Zheng, R.B.; Persson, M.; Letts, J.A.; Polakowski, R.; Bai, Y.; Borisova, S.N.; Seto, N.O.; Lowary, T.L.; Palcic, M.M.; Evans, S.V.
    ABO(H) blood group A and B glycosyltransferases recognize substrate via specific conformational changes (2008), J. Biol. Chem., 283, 10097-10108.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of truncated wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant GTA/GTB mutant chimeric enzymes, complexing with synthetic antigen disaccharides or UDP, hanging drop vapour diffusion method, different solutions for the different chimeric mutants, X-ray diffraction structure determinationand analysis at 1.41-1.75 A resolution, overview, structure modelling Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of GTA/GTB chimeric enzymes GTB/G176R and GTB/G176R/G235S Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of recombinant wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme catalyzes the final step in the enzymatic synthesis of the ABO(H) blood group A antigen, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P16442
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes from Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyzes the final step in the enzymatic synthesis of the ABO(H) blood group A antigen, overview Homo sapiens ?
-
?
additional information substrate binding structure by wild-type and mutant GTA, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More structure analysis of GTA/GTB chimeric enzymes GTB/G176R and GTB/G176R/G235S bound to a panel of donor and acceptor analogue substrates, open, semi-closed, and closed conformations, overview Homo sapiens

Synonyms

Synonyms Comment Organism
ABO(H) blood group A glycosyltransferase
-
Homo sapiens
alpha-(1,3)-N-acetylgalactosaminyltransferase
-
Homo sapiens
GTA
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Homo sapiens