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Literature summary for 2.4.1.40 extracted from

  • Takeya, A.; Hosomi, O.; Ishiura, M.
    Complete purification and characterization of alpha-3-N-acetylgalactosaminyltransferase encoded by the human blood group A gene (1990), J. Biochem., 107, 360-368.
    View publication on PubMed
Search for more literature for 2.4.1.40

Inhibitors

Inhibitors Comment Organism Structure
2'-fucosyllactose substrate inhibition above 1 mM Homo sapiens
lacto-N-fucopentaose I substrate inhibition above 2 mM Homo sapiens
UDP
-
 Homo sapiens
UDP-galactose
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 UDP-GalNAc
-
 Homo sapiens
0.27
-
 2'-fucosyllactose
-
 Homo sapiens
0.35
-
 lacto-N-fucopentaose I
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE with and without 2-mercaptoethanol Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 blood group A
-

Purification (Commentary)

Purification (Comment) Organism
blood group A Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma A Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, rapid loss of activity Homo sapiens
-80°C, 20% loss of activity after 1 year Homo sapiens
4°C, 0.05 M Tris-HCl, pH 7.4, 2 mM MnCl2, 1 mM EDTA, 0.01% w/v Triton X-100, 0.2 M NaCl, 1 mM dithiothreitol, 0.03% w/v NaN3, 10% loss of activity per month Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme also catalyzes the transfer of galactose in alpha-linkage to 2'-fucosyllactose, the transfer rate of galactose is much lower than that of N-acetylgalactosamine Homo sapiens ?
-
 ?
UDP-N-acetyl-D-galactosamine + 2'-fucosyllactose
-
 Homo sapiens UDP + N-acetyl-alpha-D-galactosaminyl-2'-fucosyllactose
-
 ?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
-
 Homo sapiens UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
 ?
UDP-N-acetyl-D-galactosamine + lacto-N-fucopentaose I i.e. Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc Homo sapiens UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[lacto-N-fucopentaose I]
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE with and without 2-mercaptoethanol Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 30 min, 5% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
 Homo sapiens