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Literature summary for 2.4.1.40 extracted from

  • Seto, N.O.L.; Palcic, M.M.; Compston, C.A.; Li, H.; Bundle, D.R.; Narang, S.A.
    Sequential interchange of four amino acids from blood group B to blood group A glycosyltransferase boosts catalytic activity and progressively modifies substrate recognition in human recombinant enzymes (1997), J. Biol. Chem., 272, 14133-14138.
    View publication on PubMed
Search for more literature for 2.4.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TG-1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens
0.015
-
 UDP-GalNAc
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-Gal + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl-D-galactosamine + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens UDP + alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
-
 ?

Synonyms

Synonyms Comment Organism
GTA
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000333
-
 UDP-Gal
-
 Homo sapiens
0.0817
-
 UDP-GalNAc
-
 Homo sapiens