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Literature summary for 2.4.1.40 extracted from

  • Marcus, S.L.; Polakowski, R.; Seto, N.O.L.; Leinala, E.; Borisova, S.; Blancher, A.; Roubinet, F.; Evans, S.V.; Palcic, M.M.
    A single point mutation reverses the donor specificity of human blood group B-synthesizing galactosyltransferase (2003), J. Biol. Chem., 278, 12403-12405.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21, P234S-mutant of blood-group substance B-dependent galactosyltransferase Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
G176R/P234S/S235G/M266L/A268G-mutant with and without H-antigen, at 1.55 and 1.65 A resolution respectively Homo sapiens
of catalytic domain residues 63-354, with and without L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3-acceptor and UDP, at 1.35 and 1.8 A resolution respectively Homo sapiens

Protein Variants

Protein Variants Comment Organism
G176R/P234S/S235G/M266L/A268G expressed in Escherichia coli BL21, although 4 of the mutations correspond to a change from A- to B-blood group, the P234S-mutation shows a remarkable increase in A-donor specificity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
Homo sapiens
0.0099
-
UDP-GalNAc
-
Homo sapiens
0.144
-
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
Homo sapiens
0.167
-
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 G176R/P234S/S235G/M266L/A268G mutant Homo sapiens
0.242
-
UDP-GlcNAc
-
Homo sapiens
3.74
-
UDP-GalNAc G176R/P234S/S235G/M266L/A268G mutant Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P16442 only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants Homo sapiens ?
-
?
UDP-N-acetyl-D-galactosamine + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
Homo sapiens UDP + alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
-
?
UDP-N-acetyl-D-glucosamine + L-fucosyl-alpha-1,2-beta-glucosyl-O(CH2)7CH3
-
Homo sapiens UDP + N-acetyl-alpha-D-glucosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-glucosyl-O(CH2)7CH3
-
?

Synonyms

Synonyms Comment Organism
GTA
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
UDP-GalNAc G176R/P234S/S235G/M266L/A268G mutant Homo sapiens
0.292
-
UDP-GalNAc wild-type Homo sapiens