Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21, P234S-mutant of blood-group substance B-dependent galactosyltransferase | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
G176R/P234S/S235G/M266L/A268G-mutant with and without H-antigen, at 1.55 and 1.65 A resolution respectively | Homo sapiens |
of catalytic domain residues 63-354, with and without L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3-acceptor and UDP, at 1.35 and 1.8 A resolution respectively | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G176R/P234S/S235G/M266L/A268G | expressed in Escherichia coli BL21, although 4 of the mutations correspond to a change from A- to B-blood group, the P234S-mutation shows a remarkable increase in A-donor specificity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0087 | - |
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 | - |
Homo sapiens | |
0.0099 | - |
UDP-GalNAc | - |
Homo sapiens | |
0.144 | - |
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 | - |
Homo sapiens | |
0.167 | - |
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 | G176R/P234S/S235G/M266L/A268G mutant | Homo sapiens | |
0.242 | - |
UDP-GlcNAc | - |
Homo sapiens | |
3.74 | - |
UDP-GalNAc | G176R/P234S/S235G/M266L/A268G mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16442 | only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 | - |
Homo sapiens | UDP + alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3 | - |
? | |
UDP-N-acetyl-D-glucosamine + L-fucosyl-alpha-1,2-beta-glucosyl-O(CH2)7CH3 | - |
Homo sapiens | UDP + N-acetyl-alpha-D-glucosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-glucosyl-O(CH2)7CH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GTA | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
UDP-GalNAc | G176R/P234S/S235G/M266L/A268G mutant | Homo sapiens | |
0.292 | - |
UDP-GalNAc | wild-type | Homo sapiens |