Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-lactalbumin | stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-lactalbumin | inhibits reaction with UDPgalactose and N-acetylglucosamine | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
UDPgalactose | - |
Bos taurus | |
2.8 | - |
agalactokeratan | - |
Bos taurus | |
21 | - |
D-glucose | - |
Bos taurus | |
40 | - |
N-acetylglucosamine | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation at 14.9% of the activity with Mn2+ | Bos taurus | |
Mn2+ | required | Bos taurus | |
Mn2+ | maximal activity at 4 mM | Bos taurus | |
Mn2+ | Km for MnCl2: 0.34 mM | Bos taurus | |
Zn2+ | activation at 9.2% of the activity with Mn2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42200 | - |
x * 42200, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides | Bos taurus | biosynthesis of keratan sulfate-like polysaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cornea | - |
Bos taurus | - |
milk | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.7 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + methyl 2-acetamido-2-deoxy-beta-D-glucoside | 76% of the activity with N-acetylglucosamine | Bos taurus | UDP + beta-D-galactosyl-1,4-beta-1-O-methyl-2-deoxy-2-acetylamido-beta-D-glucopyranoside | - |
? | |
UDP-galactose + methyl 2-bromo-acetamido-2-deoxy-beta-D-glucoside | 75% of the activity with N-acetylglucosamine | Bos taurus | UDP + beta-D-galactosyl-1,4-1-O-methyl-2-bromo-acetamido-2-deoxy-beta-D-glucoside | - |
? | |
UDP-galactose + methyl 2-deoxy-2-benzamido-beta-D-glucoside | 16% of the activity with N-acetylglucosamine | Bos taurus | UDP + beta-D-galactosyl-1,4-1-O-methyl-2-deoxy-2-benzamido-beta-D-glucoside | - |
? | |
UDPgalactose + 2-acetamido-N-(L-aspart-4-oyl)-1,2-dideoxy-beta-glucoside | 65% of the activity with N-acetylglucosamine | Bos taurus | ? | - |
? | |
UDPgalactose + agalacto-ovomucoid | 65% of the activity with N-acetylglucosamine | Bos taurus | ? | - |
? | |
UDPgalactose + agalactokeratan | agalactokeratan from bovine cornea and nasal septum, at 5% and 13% of the activity with N-acetylglucosamine | Bos taurus | ? | - |
? | |
UDPgalactose + asialo-agalacto-alpha1-glycoprotein | 42% of the activity with N-acetylglucosamine | Bos taurus | ? | - |
? | |
UDPgalactose + di-acetylchitobiose | 54% of the activity with N-acetylglucosamine | Bos taurus | ? | - |
? | |
UDPgalactose + glucose | in presence of alpha-lactalbumin | Bos taurus | lactose + UDP | - |
? | |
UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide | glycoproteins containing terminal nonreducing N-acetylglucosaminyl units | Bos taurus | UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide | - |
? | |
UDPgalactose + N-acetylglucosamine | - |
Bos taurus | UDP + N-acetyllactosamine | - |
? | |
UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides | biosynthesis of keratan sulfate-like polysaccharides | Bos taurus | ? | - |
? | |
UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside | 67% of the activity with N-acetylglucosamine | Bos taurus | ? | - |
? | |
UDPgalactose + tri-N-acetylchitotriose | 64% of the activity with N-acetylglucosamine | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42200, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | - |
Bos taurus |