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Literature summary for 2.4.1.25 extracted from
- One-pot synthesis of cycloamyloses from sucrose by dual enzyme treatment: combined reaction of amylosucrase and 4-alpha-glucanotransferase (2011), J. Agric. Food Chem., 59, 5044-5051.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | development of an efficient biocatalytic production process of cycloamyloses directly from sucrose by use of Synechocystis sp. 4-alpha-glucanotransferase and Neisseria polysaccharea amylosucrase. From one-pot synthesis, the maximum cycloamylose yield of 9.6%, w/w with 0.3 M sucrose is achieved with 10 units/ml of amylosucrase and 0.1 unit/ml of 4-alpha-glucanotransferase at 40°C for a 3 h reaction in a simultaneous dual enzyme reaction mode. The size of linear alpha-(1,4)-glucan is positively related to the cycloamylose productivity by 4-alpha-glucanotransferase in a hyperbolic manner. The dual enzyme reaction converts sucrose directly to cycloamyloses via in situ transient linear alpha-(1,4)-glucan as an soluble intermediate | Synechocystis sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechocystis sp. | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4alphaGTase | - |
Synechocystis sp. |
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Release 2023.1 (January 2023)
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