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Literature summary for 2.4.1.25 extracted from

  • Lee, B.H.; Oh, D.K.; Yoo, S.H.
    Characterization of 4-alpha-glucanotransferase from Synechocystis sp. PCC 6803 and its application to various corn starches (2009), N. Biotechnol., 26, 29-36.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged alpaGTase in Escherichia coli strain BL21 (DE3) Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, recombinant enzyme, SDS-PAGE Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
strain PCC 6803, ATCC 27184#
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged alpaGTase 2.9fold from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.84
-
purified recombinant His-tagged enzyme Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme displays transglycosylating activity on various maltooligosaccharides, e.g. corn starches consisting of different proportions of amylopectin and amylose, of which the smallest donor and acceptor molecules are determined to be maltose and glucose, respectively, overview. Activity of alphaGTase decreases amylopectin and increases cycloamylose contents in the polymers, product determination by MALDI-TOF-MS analysis, product molecular weight and branch-chain distribution after isoamylolysis of different starches, overview Synechocystis sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 57000, recombinant enzyme, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
alphaGTase
-
Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
recombinant enzyme Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant enzyme Synechocystis sp.