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Literature summary for 2.4.1.25 extracted from
- Cloning and expression of the 4-alpha-glucanotransferase gene from the hyperthermophilic archaeon Pyrococcus sp. KOD1, and characterization of the enzyme (1997), J. Ferment. Bioeng., 83, 540-548.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gtpK cloned, sequenced and overexpressed in Escherichia coli | Pyrococcus sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76690 | - |
calculated from cDNA | Pyrococcus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltopentaose + maltopentaose | Pyrococcus furiosus | metabolism of starch in the bacterium | maltooligosaccharides | - |
? |  |
| maltopentaose + maltopentaose | Dictyoglomus thermophilum | metabolism of starch in the bacterium | maltooligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyoglomus thermophilum | - |
- |
- |
| Pyrococcus furiosus | - |
- |
- |
| Pyrococcus sp. | - |
KOD1 | - |
| Pyrococcus sp. KOD1 | - |
KOD1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltohexaose + maltotriose | - |
Pyrococcus sp. | maltodextrins | - |
? | |
| maltohexaose + maltotriose | - |
Pyrococcus sp. KOD1 | maltodextrins | - |
? | |
| maltohexaose + maltotriose | - |
Pyrococcus sp. | D-glucose + maltooligosaccharides | - |
? | |
| maltopentaose + maltopentaose | metabolism of starch in the bacterium | Pyrococcus furiosus | maltooligosaccharides | - |
? | |
| maltopentaose + maltopentaose | metabolism of starch in the bacterium | Dictyoglomus thermophilum | maltooligosaccharides | - |
? | |
| maltopentaose + maltotriose | - |
Pyrococcus sp. | maltodextrins | - |
? | |
| maltopentaose + maltotriose | - |
Pyrococcus sp. KOD1 | maltodextrins | - |
? | |
| maltopentaose + maltotriose | - |
Pyrococcus sp. | D-glucose + maltooligosaccharides | - |
? | |
| maltose + maltose | - |
Pyrococcus sp. | maltotriose + glucose | - |
? | |
| maltose + maltose | - |
Pyrococcus sp. KOD1 | maltotriose + glucose | - |
? | |
| maltotetraose + maltotriose | - |
Pyrococcus sp. | maltodextrins | - |
? | |
| maltotetraose + maltotriose | - |
Pyrococcus sp. KOD1 | maltodextrins | - |
? | |
| maltotetraose + maltotriose | - |
Pyrococcus sp. | D-glucose + maltooligosaccharides | - |
? | |
| maltotheptaose + maltotriose | - |
Pyrococcus sp. | maltodextrins | - |
? | |
| maltotheptaose + maltotriose | - |
Pyrococcus sp. KOD1 | maltodextrins | - |
? | |
| maltotheptaose + maltotriose | - |
Pyrococcus sp. | D-glucose + maltooligosaccharides | - |
? | |
| maltotriose + maltotriose | - |
Pyrococcus sp. | maltodextrins | - |
? | |
| maltotriose + maltotriose | - |
Pyrococcus sp. | D-glucose + maltooligosaccharides | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Pyrococcus sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
stable even after heating for 30 min | Pyrococcus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Pyrococcus sp. |
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