Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | D-enzyme, slight inhibition | Pseudomonas stutzeri | |
cyclomaltohexaose | inhibition 25% | Pseudomonas stutzeri | |
dithiothreitol | D-enzyme, slight inhibition | Pseudomonas stutzeri | |
EDTA | D-enzyme, slight inhibition | Pseudomonas stutzeri | |
Fe2+ | D-enzyme, 50% inhibition | Pseudomonas stutzeri | |
Hg2+ | D-enzyme | Pseudomonas stutzeri | |
methyl-alpha-D-glucoside | competitive inhibition | Escherichia coli | |
p-chloromercuribenzoic acid | D-enzyme, 30% inhibition | Pseudomonas stutzeri | |
phenyl-beta-D-glucoside | competitive inhibition | Escherichia coli | |
Tris | D-enzyme, 50 mM, 35% inhibition | Pseudomonas stutzeri | |
Zn2+ | D-enzyme | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.1 | - |
maltotetraose | - |
Pseudomonas stutzeri | |
8.3 | - |
maltose | D-enzyme | Pseudomonas stutzeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
1 * 38000 + 1 * 47000, amylomaltase | Pseudomonas stutzeri |
47000 | - |
1 * 38000 + 1 * 47000, amylomaltase | Pseudomonas stutzeri |
52000 | - |
1 * 52000 + 1 * 63000, D-enzyme | Pseudomonas stutzeri |
63000 | - |
1 * 52000 + 1 * 63000, D-enzyme | Pseudomonas stutzeri |
71000 | - |
- |
Escherichia coli |
74000 | - |
amylomaltase, gel filtration | Pseudomonas stutzeri |
115000 | - |
D-enzyme, gel filtration | Pseudomonas stutzeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia coli ML 30 | - |
- |
- |
Pseudomonas stutzeri | - |
NRRL B3389 | - |
Pseudomonas stutzeri NRRL B3389 | - |
NRRL B3389 | - |
Solanum tuberosum | - |
potato | - |
Streptococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
both amylomaltase and D-enzyme | Pseudomonas stutzeri |
E. coli ML 30, partially | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, stored as a suspension in 3.2 M ammonium sulfate, no significant loss of activity for at least 1 month | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + maltotriose | D-enzyme | Pseudomonas stutzeri | maltononaose + D-glucose | - |
? | |
maltohexaose + maltodextrin | only amylomaltase, no polymers larger than the initial maltodextrin substrate, maltohexaose is a good donor substrate, but unable to function as an acceptor | Pseudomonas stutzeri | D-glucose + maltopentaose | - |
? | |
maltononaose + maltotriose | D-enzyme | Pseudomonas stutzeri | maltoundecaose + D-glucose | - |
? | |
maltopentaose + maltodextrin | - |
Pseudomonas stutzeri | maltose + higher dextrins | - |
? | |
maltopentaose + maltotriose | D-enzyme | Pseudomonas stutzeri | maltoheptaose + D-glucose | - |
? | |
maltopentaose + maltotriose | amylomaltose | Pseudomonas stutzeri | homologous 1,4-alpha-D-glucans | - |
? | |
maltose + maltose | - |
Escherichia coli | maltotriose + glucose | - |
? | |
maltose + maltose | D-enzyme | Pseudomonas stutzeri | maltotriose + glucose | - |
? | |
maltose + maltose | D-enzyme | Pseudomonas stutzeri NRRL B3389 | maltotriose + glucose | - |
? | |
maltose + maltose | - |
Escherichia coli ML 30 | maltotriose + glucose | - |
? | |
maltotetraose + maltodextrin | - |
Pseudomonas stutzeri | maltose + higher dextrins | - |
? | |
maltotetraose + maltotriose | D-enzyme | Pseudomonas stutzeri | glucose + maltoheptaose + maltodecaose | - |
? | |
maltotetraose + maltotriose | amylomaltose | Pseudomonas stutzeri | homologous 1,4-alpha-glucans | - |
? | |
maltotetraose + maltotriose | - |
Solanum tuberosum | D-glucose + maltooligosaccharides | - |
? | |
maltotetraose + maltotriose | amylomaltose, most active with maltotetraose | Pseudomonas stutzeri | D-glucose + maltooligosaccharides | - |
? | |
maltotriose + maltodextrin | - |
Pseudomonas stutzeri | maltose + higher dextrins | - |
? | |
maltotriose + maltodextrin | - |
Pseudomonas stutzeri NRRL B3389 | maltose + higher dextrins | - |
? | |
maltotriose + maltodextrin | amylomaltase | Pseudomonas stutzeri | maltopentaose + ? | - |
? | |
maltotriose + maltodextrin | amylomaltase | Pseudomonas stutzeri NRRL B3389 | maltopentaose + ? | - |
? | |
maltotriose + maltotriose | D-enzyme | Pseudomonas stutzeri | maltopentaose + D-glucose | - |
? | |
maltotriose + maltotriose | D-enzyme | Pseudomonas stutzeri | D-glucose + maltopentaose + maltoheptaose + maltononaose + maltoundecaose | - |
? | |
maltotriose + maltotriose | amylomaltose | Pseudomonas stutzeri | homologous alpha-1,4-D-glucans | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | maltose is not a donor substrate, only weak acceptor activity | Solanum tuberosum | ? | - |
? | |
additional information | D-enzyme, maltohexaose is no initial substrate | Pseudomonas stutzeri | ? | - |
? | |
additional information | 2 glycosyltransferases, amylomaltase and D-enzyme | Pseudomonas stutzeri | ? | - |
? | |
additional information | D-enzyme, maltohexaose is no initial substrate | Pseudomonas stutzeri NRRL B3389 | ? | - |
? | |
additional information | 2 glycosyltransferases, amylomaltase and D-enzyme | Pseudomonas stutzeri NRRL B3389 | ? | - |
? | |
additional information | - |
Escherichia coli ML 30 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 38000 + 1 * 47000, amylomaltase | Pseudomonas stutzeri |
dimer | 1 * 52000 + 1 * 63000, D-enzyme | Pseudomonas stutzeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
D-enzyme | Pseudomonas stutzeri |
37 | - |
amylomaltase | Pseudomonas stutzeri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 42 | D-enzyme, activity decreases rapidly above 37°C and is almost completely lost at 42°C | Pseudomonas stutzeri |
50 | - |
amylomaltase, rapidly inactivated at temperatures above | Pseudomonas stutzeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Escherichia coli |
7.6 | 7.7 | both amylomaltase and D-enzyme | Pseudomonas stutzeri |