Cloned (Comment) | Organism |
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FFT phylogenetic tree | Lolium perenne |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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additional information | Lolium perenne | Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose | ? | - |
? | |
additional information | Lolium perenne Bravo | Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
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Lolium perenne | Q8S337 | - |
- |
Lolium perenne Bravo | Q8S337 | - |
- |
Source Tissue | Comment | Organism | Textmining |
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leaf | elongating leaves, basal segment | Lolium perenne | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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1-kestose + sucrose | - |
Lolium perenne | 1,1-kestotetraose + 1,6-kestotetraose | neither this fructan nor the subsequent mixed series is found in vivo in the tissues of Lolium perenne | ? | |
1-kestose + sucrose | - |
Lolium perenne Bravo | 1,1-kestotetraose + 1,6-kestotetraose | neither this fructan nor the subsequent mixed series is found in vivo in the tissues of Lolium perenne | ? | |
1-kestose + sucrose | - |
Lolium perenne | 6G,6-kestotetraose | - |
? | |
1-kestose + sucrose | - |
Lolium perenne Bravo | 6G,6-kestotetraose | - |
? | |
additional information | Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose | Lolium perenne | ? | - |
? | |
additional information | both 1-kestotriose and 6G-kestotriose acts as fructosyl acceptors, producing 1- and 6-kestotetraose (bifurcose) and 6G,6-kestotetraose, respectively. 1-Kestotriose is not a fructosyl donor since incubation with 1-kestotriose alone does not lead to the synthesis of a 6-linked fructan. Bifurcose formation ceases and 6G,6-kestotetraose is formed instead, when recombinant fructan:fructan 6G-fructosyltransferase, 6G-FFT, is introduced in the enzyme assay with sucrose and 1-kestotriose as substrates | Lolium perenne | ? | - |
? | |
additional information | Lolium perenne plants produce a majority of levan neoseries built on the 6G-kestotriose backbone, incubation with both sucrose and 6G-kestotriose allow the synthesis of 6G,6-kestotetraose | Lolium perenne Bravo | ? | - |
? | |
additional information | both 1-kestotriose and 6G-kestotriose acts as fructosyl acceptors, producing 1- and 6-kestotetraose (bifurcose) and 6G,6-kestotetraose, respectively. 1-Kestotriose is not a fructosyl donor since incubation with 1-kestotriose alone does not lead to the synthesis of a 6-linked fructan. Bifurcose formation ceases and 6G,6-kestotetraose is formed instead, when recombinant fructan:fructan 6G-fructosyltransferase, 6G-FFT, is introduced in the enzyme assay with sucrose and 1-kestotriose as substrates | Lolium perenne Bravo | ? | - |
? |
Synonyms | Comment | Organism |
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6G-FFT | - |
Lolium perenne |
fructan:fructan 6G-fructosyltransferase | - |
Lolium perenne |
General Information | Comment | Organism |
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metabolism | the enzyme is required for fructan biosynthesis. In the second step, a 6G-FFT produces 6G-kestotriose, using 1-kestotriose as fructosyl donor and sucrose as fructosyl acceptor. In further steps, higher polymeric fructans are synthesized by a 1-FFT activity through beta(2-1)-linked chain elongation or by a 6-FT activity through beta(2-6)-linked chain elongation.The 6G-FFT from Lolium perenne also has 1-FFT activity | Lolium perenne |