Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.243 extracted from

  • Lasseur, B.; Lothier, J.; Djoumad, A.; De Coninck, B.; Smeekens, S.; Van Laere, A.; Morvan-Bertrand, A.; Van den Ende, W.; Prudhomme, M.P.
    Molecular and functional characterization of a cDNA encoding fructan:fructan 6G-fructosyltransferase (6G-FFT)/fructan:fructan 1-fructosyltransferase (1-FFT) from perennial ryegrass (Lolium perenne L.) (2006), J. Exp. Bot., 57, 2719-2734.
    View publication on PubMed
Search for more literature for 2.4.1.243

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Pichia pastoris Lolium perenne

Organism

Organism UniProt Comment Textmining
Lolium perenne Q8S337 cv. Bravo
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf the enzyme is predominantly expressed in the basal part of elongating leaves and leaf sheaths Lolium perenne
-
additional information highly expressed in photosynthetically active tissues Lolium perenne
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme possesses both fructan:fructan 6G-fructosyltransferase and fructan:fructan 1-fructosyltransferase activities Lolium perenne ?
-
 ?
sucrose + sucrose
-
 Lolium perenne 1-kestotriose + ?
-
 ?

Synonyms

Synonyms Comment Organism
6G-FFT
-
 Lolium perenne
fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase
-
 Lolium perenne

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Lolium perenne

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 20°C: about 80% of maximal activity, 40°C: about 80% of maximal activity Lolium perenne

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
 Lolium perenne

pH Range

pH Minimum pH Maximum Comment Organism
5 6 pH 5.0: about 80% of maximal activity, pH 6.0: about 90% of maximal activity, pH 4.3: about 20% of maximal activity, pH 6.5: about 20% of maximal activity Lolium perenne