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Literature summary for 2.4.1.19 extracted from
- Mutation of tyrosine167histidine at remote substrate binding subsite -6 in alpha-cyclodextrin glycosyltransferase enhancing alpha-cyclodextrin specificity by directed evolution (2014), Mol. Biotechnol., 56, 232-239.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Paenibacillus macerans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y167H | the mutations increases the alpha:beta ratio in cyclodextrin product mixture from 3.4 to 7.8 in comparison with the wild type enzyme | Paenibacillus macerans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Paenibacillus macerans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| soluble starch + glycosyl acceptor | Paenibacillus macerans | - |
cyclodextrins | alpha-cyclodextrin is the main product | ? |  |
| soluble starch + glycosyl acceptor | Paenibacillus macerans 602-1 | - |
cyclodextrins | alpha-cyclodextrin is the main product | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus macerans | - |
- |
- |
| Paenibacillus macerans 602-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paenibacillus macerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | alpha-cyclodextrin is the main product | ? | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans 602-1 | cyclodextrins | alpha-cyclodextrin is the main product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Paenibacillus macerans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-CGTase | - |
Paenibacillus macerans |
| alpha-cyclodextrin glycosyltransferase | - |
Paenibacillus macerans |
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Release 2023.1 (January 2023)
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