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Literature summary for 2.4.1.19 extracted from

  • Li, Z.; Huang, M.; Gu, Z.; Holler, T.P.; Cheng, L.; Hong, Y.; Li, C.
    Asp577 mutations enhance the catalytic efficiency of cyclodextrin glycosyltransferase from Bacillus circulans (2016), Int. J. Biol. Macromol., 83, 111-116.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in in Bacillus subtilis strain WB600 Niallia circulans

Protein Variants

Protein Variants Comment Organism
D577A the mutation increases the beta-cyclization activity of the enzyme with 23% higher catalytic efficiency compared to the wild type. The mutation also increases the affinity for maltodextrin Niallia circulans
D577G the mutation increases the beta-cyclization activity of the enzyme with 43.9% higher catalytic efficiency compared to the wild type. The mutation also increases the affinity for maltodextrin Niallia circulans
D577I the mutation decreases the beta-cyclization activity of the enzyme Niallia circulans
D577L the mutation decreases the beta-cyclization activity of the enzyme Niallia circulans
D577V the mutation decreases the beta-cyclization activity of the enzyme Niallia circulans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans STB01
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltodextrin + glycosyl acceptor
-
Niallia circulans cyclodextrins
-
?
maltodextrin + glycosyl acceptor
-
Niallia circulans STB01 cyclodextrins
-
?
soluble starch + glycosyl acceptor
-
Niallia circulans cyclodextrins
-
?
soluble starch + glycosyl acceptor
-
Niallia circulans STB01 cyclodextrins
-
?

Synonyms

Synonyms Comment Organism
beta-CGTase
-
Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
371
-
maltodextrin mutant enzyme D577G, at pH 6.5 and 50°C Niallia circulans
403
-
maltodextrin wild type enzyme, at pH 6.5 and 50°C Niallia circulans
406
-
maltodextrin mutant enzyme D577A, at pH 6.5 and 50°C Niallia circulans
467
-
maltodextrin mutant enzyme D577V, at pH 6.5 and 50°C Niallia circulans
513
-
maltodextrin mutant enzyme D577I, at pH 6.5 and 50°C Niallia circulans
583
-
maltodextrin mutant enzyme D577L, at pH 6.5 and 50°C Niallia circulans