Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.19 extracted from

  • Costa, H.; Distefano, A.J.; Marino-Buslje, C.; Hidalgo, A.; Berenguer, J.; Biscoglio de Jimenez Bonino, M.; Ferrarotti, S.A.
    The residue 179 is involved in product specificity of the Bacillus circulans DF 9R cyclodextrin glycosyltransferase (2012), Appl. Microbiol. Biotechnol., 94, 123-130.
    View publication on PubMed
Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Niallia circulans

Protein Variants

Protein Variants Comment Organism
additional information position 179 is involved in enzyme product specificity and must be occupied by Gly or by amino acid residues able to interact with the substrate through hydrogen bonds in a way that the cyclization process occurs efficiently Niallia circulans
Q179G mutation to residue G which is conserved in all the corresponding enzymes except in that from Bacillus circulans Df 9R. Activity and kinetic parameters remain unchanged Niallia circulans
Q179L mutation results in a different ratio of cyclodextrin products with a ratio for alpha- to beta- to gamma-cyclodextrin 1:1.7:0.7, a lower catalytic efficiency, and a decreased ability to convert starch into cyclodextrins Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km value is 0.69 mg/ml for wild-type, 0.57 mg/ml for mutant Q179G, 0.54 mg/ml for mutant Q179l, substrate soluble potato starch, pH 6.4, 56°C Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans DF 9R
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble potato starch
-
 Niallia circulans alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin ratio for alpha- to beta- to gamma-cyclodextrin 1:1.3:0.5 ?
soluble potato starch
-
 Niallia circulans DF 9R alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin ratio for alpha- to beta- to gamma-cyclodextrin 1:1.3:0.5 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
127.5
-
 soluble potato starch mutant Q179L, pH 6.4, 56°C, calculated as production of beta-cyclodextrin Niallia circulans
246.2
-
 soluble potato starch wild-type, pH 6.4, 56°C, calculated as production of beta-cyclodextrin Niallia circulans
247.2
-
 soluble potato starch mutant Q179G, pH 6.4, 56°C, calculated as production of beta-cyclodextrin Niallia circulans