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Literature summary for 2.4.1.19 extracted from
- A novel cyclodextrin glycosyltransferase from Bacillus sphaericus strain 41: production, characterization and catalytic properties (2009), Biochem. Eng. J., 48, 124-131.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | CGTase is an important industrial enzyme, unique in its capacity to convert starch and related substrates into cyclodextrins through cyclization, an intramolecular transglycosylation reaction | Lysinibacillus sphaericus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-cyclodextrin | strong product inhibition of CGTase | Lysinibacillus sphaericus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, overview | Lysinibacillus sphaericus | |
| 0.8 | - |
maltodextrin | pH 6.0, 65°C | Lysinibacillus sphaericus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Lysinibacillus sphaericus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
x * 59000, SDS-PAGE | Lysinibacillus sphaericus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | - |
alkalophilic strain 41, isolated from soybean-soil culture | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 315fold by ammonium sulfate fractionation, beta-cyclodextrin affinity chromatography, and ultrafiltration | Lysinibacillus sphaericus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23.5 | - |
coupling activity, purified enzyme | Lysinibacillus sphaericus |
| 75.8 | - |
cyclization activity, purified enzyme | Lysinibacillus sphaericus |
| 198.6 | - |
disproportionation activity, purified enzyme | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methanol + maltodextrin | - |
Lysinibacillus sphaericus | alpha-methyl-D-glucopyranoside + beta-cyclodextrin | - |
? | |
| additional information | in addition to the intramolecular transglycosylation and cyclization producing beta-cyclodextrins, using maltodextrin and starch as substrates, the CGTase shows disproportionation and coupling activities, intermolecular transglycosylation reactions. The enzyme produces alpha-, beta-, and gamma-cyclodextrins in the ratio of 0.40:1:0.45. Activity with different starch types, overview | Lysinibacillus sphaericus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CGTase | - |
Lysinibacillus sphaericus |
| cyclodextrin glycosyltransferase | - |
Lysinibacillus sphaericus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
cyclization activity | Lysinibacillus sphaericus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 80 | activity range, about 35% of maximal activity at 35°C and 80°C | Lysinibacillus sphaericus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 65 | purified native enzyme, 80% remaining activity at 35-65°C, pH 8.0, after 4 h, thermal deactivation above 70°C | Lysinibacillus sphaericus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
cyclization activity | Lysinibacillus sphaericus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | activity range, about 20% of maximal activity at pH 4.5, and 40% at pH 10.0 | Lysinibacillus sphaericus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
purified native enzyme, 50°C, 4 h, 34.2% retaining activity | Lysinibacillus sphaericus |
| 6 | 7 | purified native enzyme, 50°C, 4 h, almost completely stable | Lysinibacillus sphaericus |
| 8 | 10 | purified native enzyme, 50°C, 4 h, 16.8% retaining activity | Lysinibacillus sphaericus |
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