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Literature summary for 2.4.1.19 extracted from

  • Li, Z.; Gu, Z.; Wang, M.; Du, G.; Wu, J.; Chen, J.
    Delayed supplementation of glycine enhances extracellular secretion of the recombinant alpha-cyclodextrin glycosyltransferase in Escherichia coli (2010), Appl. Microbiol. Biotechnol., 85, 553-561.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression and extracellular secretion of the recombinant alpha-cyclodextrin glycosyltransferase in Escherichia coli strain BL21(DE3), promotable by glycine, when supplemented optimally at the middle of the exponential growth phase at 1%, the enzyme production is increased by 11fold, glycine supplementation at the beginning of cell growth achieves a 1.2fold activation, overview. Glycine increases the cell membrane permeability, mechanism, overview Paenibacillus macerans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
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Paenibacillus macerans
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additional information no alpha-CGTase activity in the soluble cytoplasmic fraction, very low activity in the periplasmic fraction Paenibacillus macerans
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Paenibacillus macerans CGTase is an extracellular enzyme capable of converting starch or starch derivatives into cyclodextrins through an intramolecular transglycosylation reaction. Cyclodextrins are cyclic, nonreducing oligoglucopyranose molecules linked via alpha(1,4)-glycosidic bonds ?
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?
additional information Paenibacillus macerans JFB05-01 CGTase is an extracellular enzyme capable of converting starch or starch derivatives into cyclodextrins through an intramolecular transglycosylation reaction. Cyclodextrins are cyclic, nonreducing oligoglucopyranose molecules linked via alpha(1,4)-glycosidic bonds ?
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?

Organism

Organism UniProt Comment Textmining
Paenibacillus macerans
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Paenibacillus macerans JFB05-01
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CGTase is an extracellular enzyme capable of converting starch or starch derivatives into cyclodextrins through an intramolecular transglycosylation reaction. Cyclodextrins are cyclic, nonreducing oligoglucopyranose molecules linked via alpha(1,4)-glycosidic bonds Paenibacillus macerans ?
-
?
additional information CGTase is an extracellular enzyme capable of converting starch or starch derivatives into cyclodextrins through an intramolecular transglycosylation reaction. Cyclodextrins are cyclic, nonreducing oligoglucopyranose molecules linked via alpha(1,4)-glycosidic bonds Paenibacillus macerans JFB05-01 ?
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?

Synonyms

Synonyms Comment Organism
alpha-CGTase
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Paenibacillus macerans
alpha-cyclodextrin glycosyltransferase
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Paenibacillus macerans
CGTase
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Paenibacillus macerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
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assay at Paenibacillus macerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
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assay at Paenibacillus macerans