Literature summary for 2.4.1.19 extracted from

Thiemann, V.; Donges, C.; Prowe, S.G.; Sterner, R.; Antranikian, G.
Characterisation of a thermoalkali-stable cyclodextrin glycosyltransferase from the anaerobic thermoalkaliphilic bacterium Anaerobranca gottschalkii (2004), Arch. Microbiol., 182, 226-235.

Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Anaerobranca gottschalkii

General Stability

General Stability	Organism
Ca2+ stabilizes against thermal inactivation, 5 mM CaCl2 shifts the apparent melting point from 60°C to 69°C	Anaerobranca gottschalkii
high substrate concentrations stabilize against thermal inactivation	Anaerobranca gottschalkii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
78000	-	x * 78000, SDS-PAGE	Anaerobranca gottschalkii

Organism

Organism	UniProt	Comment	Textmining
Anaerobranca gottschalkii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Anaerobranca gottschalkii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
308	-	-	Anaerobranca gottschalkii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
amylopectin + glycosyl acceptor	alpha-cyclodextrin is preferentially produced. With a longer incubation period, the alpha-cyclodextrin to beta-cyclodextrin ratio declines	Anaerobranca gottschalkii	alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin	larger cyclodextrins (>8 glucose units) are formed in the initial reaction period	?
amylose + glycosyl acceptor	alpha-cyclodextrin is preferentially produced. With a longer incubation period, the alpha-cyclodextrin to beta-cyclodextrin ratio declines	Anaerobranca gottschalkii	alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin	larger cyclodextrins (>8 glucose units) are formed in the initial reaction period	?
additional information	increasing substrate concentrations (0.5%-20.0%) and glucans containing branching points (alpha-1,6-glycosidic linkages) shift the product pattern to: beta-cyclodextrin > alpha-cyclodextrin > gamma-cyclodextrin	Anaerobranca gottschalkii	?	-	?
native starch + glycosyl acceptor	alpha-cyclodextrin is preferentially produced. With a longer incubation period, the alpha-cyclodextrin to beta-cyclodextrin ratio declines	Anaerobranca gottschalkii	alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin	larger cyclodextrins (>8 glucose units) are formed in the initial reaction period	?

Subunits

Subunits	Comment	Organism
?	x * 78000, SDS-PAGE	Anaerobranca gottschalkii

Synonyms

Synonyms	Comment	Organism
CGTase	-	Anaerobranca gottschalkii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Anaerobranca gottschalkii

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
55	70	55°C: about 75% of maximal activity, 70°C: about 90% of maximal activity	Anaerobranca gottschalkii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	5 mM CaCl2 shifts the apparent melting point from 60°C to 69°C	Anaerobranca gottschalkii
69	-	5 mM CaCl2 shifts the apparent melting point from 60°C to 69°C	Anaerobranca gottschalkii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	9	-	Anaerobranca gottschalkii

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	10	pH 5.0: about 70% of maximal activity, pH 10.0: about 50% of maximal activity	Anaerobranca gottschalkii

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Release 2023.1 (January 2023)