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Literature summary for 2.4.1.19 extracted from

  • Chung, H.C.; Yoon, S.H.; Lee, M.J.; Kim, M.J.; Kweon, K.S.; Lee, I.W.; Kim, J.W.; Oh, B.H.; Lee, H.S.; Spiridonova, V.A.; Park, K.H.
    Characterization of a thermostable cyclodextrin glucanotransferase isolated from Bacillus stearothermophilus ET1 (1998), J. Agric. Food Chem., 46, 952-959.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene cloned in Escherichia coli Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ thermal stability is remarkably increased by addition of calcium ions Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66800
-
-
 Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 ET1
-
Geobacillus stearothermophilus ET1
-
 ET1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + glycosyl acceptor corn starch Geobacillus stearothermophilus cyclodextrins
-
 r
soluble starch + glycosyl acceptor corn starch Geobacillus stearothermophilus ET1 cyclodextrins
-
 r
soluble starch + glycosyl acceptor
-
 Geobacillus stearothermophilus alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin product proportions 4.2: 5.9: 1 r
soluble starch + glycosyl acceptor
-
 Geobacillus stearothermophilus ET1 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin product proportions 4.2: 5.9: 1 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Geobacillus stearothermophilus